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Human platelet lysate enhances the proliferative activity of cultured human fibroblast-like cells from different tissues

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Abstract

Several studies have shown the presence of fibroblast-like cells in the stromal fraction of different tissues with a high proliferative and differentiation potential. Platelet alpha granules contain growth factors released into the environment during activation. The effects of different supplements for culture medium (human serum, bovine serum and platelet lysate) on cultured human fibroblast-like cells from bone marrow, adipose tissue, trabecular bone and dental pulp have been compared. Expression of typical stromal and hematopoietic markers was analyzed and proliferative rates were determined. Flow cytofluorometry showed a homogenous pattern in serial-passaged cells, with a high level of stromal cell-associated markers (CD13, CD90, CD105). The presence of platelet lysate in culture media increased the number of cell generations obtained regardless of cell source. This effect was serum-dependent. Cell-based therapies can benefit by the use of products from human origin for “ex vivo” expansion of multipotent cells.

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Acknowledgement

We wish to thank Isabel Plasencia, Casi Riol and Amparo Bernárdez for their excellent technical assistance.

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Authors and Affiliations

  1. Centro de Transfusión de la Comunidad Valenciana, Avenida del Cid, 65-A, 46014, Valencia, Spain

    Vicente Mirabet, Pilar Solves, Francisco Carbonell-Uberos, Amando Blanquer & Roberto Roig

  2. Fundación Hospital General Universitario de Valencia, Valencia, Spain

    Mª Dolores Miñana & Araceli Encabo

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  1. Vicente Mirabet
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  2. Pilar Solves
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  3. Mª Dolores Miñana
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  4. Araceli Encabo
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  7. Roberto Roig
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Correspondence to Vicente Mirabet.

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Mirabet, V., Solves, P., Miñana, M.D. et al. Human platelet lysate enhances the proliferative activity of cultured human fibroblast-like cells from different tissues. Cell Tissue Banking 9, 1–10 (2008). https://doi.org/10.1007/s10561-007-9048-x

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  • DOI: https://doi.org/10.1007/s10561-007-9048-x

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