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Long bone mesenchymal stem cells (Lb-MSCs): clinically reliable cells for osteo-diseases

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Abstract

Mesenchymal stem cells (MSCs) have been designated as the most reliable cells in clinics to treat osteo-diseases because of their versatile nature. MSCs, isolated from long bone (Lb-MSCs) are rarely reported and named as RIA-MSCs because of the reamer–irrigator–aspirator (RIA) device. The potential of these cells in the treatment of non-union bone fractures made them the ideal candidates to be studied for clinical practices. In this work, effect of cryopreservation on the proliferation and differentiation capabilities of long bone MSCs (Lb-MSCs) has been studied. For this purpose, Lb-MSCs were isolated via RIA device and characterized using flow cytometry and differentiation assays. Cells were cryopreserved for 3, 6 and 12 months and thereafter were characterized using differentiation assays and genetic markers specific for osteogenic, chondrogenic, and adipogenic potential quantitatively by qRT-PCR. Lb-MSCs were found expressing MSC characteristic markers defining their identity. The population doubling time (PDT) was about 2.5 ± 0.5 days and colonies appeared after 7–10 days. Differentiation potential and gene expression of 3, 6 and 12 months cryopreserved Lb-MSCs were unaltered. The results show that cryopreservation did not have an effect on the differentiation potential of human Lb-MSCs. Therefore, our work offers Lb-MSCs as clinically cells for treating osteo-diseases.

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Acknowledgements

The authors are indebted to the Research Council of the Mashhad University of Medical Sciences, Iran, for its approval and financial support of this research. This study was extracted from the Ph.D. thesis of Shirin Toosi.

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Authors and Affiliations

  1. Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Shirin Toosi, Fatemeh Kalalinia & Javad Behravan

  2. Stem Cell and Regenerative Medicine Research Group, Academic Center for Education, Culture and Research (ACECR), Khorasan Razavi Branch, Mashhad, Iran

    Hojjat Naderi-Meshkin, Ahmad Reza Bahrami, Asieh Heirani-Tabasi & Mahdi Mirahmadi

  3. Medical Genetic Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran

    Fatemeh Kalalinia

  4. Department of Orthopedic Surgery, Orthopedic and Trauma Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Mohammad Taghi Pievandi

  5. School of Biomedical Engineering, National Yang Ming University, Taipei, Taiwan

    Hossein Hosseinkhani

  6. Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad, Iran

    Javad Behravan

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  1. Shirin Toosi
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  2. Hojjat Naderi-Meshkin
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  3. Fatemeh Kalalinia
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Correspondence to Javad Behravan.

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Toosi, S., Naderi-Meshkin, H., Kalalinia, F. et al. Long bone mesenchymal stem cells (Lb-MSCs): clinically reliable cells for osteo-diseases. Cell Tissue Bank 18, 489–500 (2017). https://doi.org/10.1007/s10561-017-9652-3

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  • DOI: https://doi.org/10.1007/s10561-017-9652-3

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