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Acetylcholine Release Induced by the Volatile Anesthetic Sevoflurane in Rat Brain Cortical Slices

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Summary

  1. 1.

    We have investigated the effect of the volatile anesthetic sevoflurane on acetylcholine (ACh) release from rat brain cortical slices.

  2. 2.

    The release of [3H]-ACh into the incubation fluid was studied after labeling the tissue ACh with [methyl-3H]-choline chloride.

  3. 3.

    We observed that sevoflurane induced an increase on the release of ACh that was dependent on incubation time and anesthetic concentration. The sevoflurane-induced ACh release was not blocked by tetrodotoxin (TTX) and therefore was independent of sodium channels. In addition, the sevoflurane effect was not blocked by ethylene glycol-bis(β-aminoethyl ether (EGTA) or cadmium (Cd2+), thus independent of extracellular calcium.

  4. 4.

    The sevoflurane-induced ACh release was inhibited by 1,2-bis (2-aminophenoxy) ethane-N,N,N’,N’-tetra-acetic acid (BAPTA-AM), suggestingthe involvement of intracellular calcium-sensitive stores in the process. Dantrolene, an inhibitor of ryanodine receptors, had no effect but 2-aminoethoxydiphenylborate (2-APB), a membrane-permeable inhibitor of inositol 1,4,5-triphosphate receptor inhibited the sevoflurane-induced release of ACh.

  5. 5.

    It is concluded that sevoflurane-induced release of ACh in brain cortical slices involves the mobilization of calcium from IP_{3}-sensitive calcium stores.

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Authors and Affiliations

  1. Departamento de Morfologia, ICB-UFMG, Belo Horizonte-Minas Gerais, Brazil

    Janice H. Silva

  2. Departamento de Cirurgia, Faculdade de Medicina da UFMG, Belo Horizonte-Minas Gerais, Brazil

    Renato S. Gomez & Cristina Guatimosim

  3. Laboratório de Neurofarmacologia, Departamento de Farmacologia, ICB-UFMG, Belo Horizonte-Minas Gerais, Brazil

    Ana Cristina N. Pinheiro & Marcus V. Gomez

  4. To whom correspondence should be addressed at Departamento de Farmacologia-ICB-UFMG, Av. Antonio Carlos 6627-Pampulha, CEP: 31270-901, Belo Horizonte-Minas Gerais, Brazil

    Marcus V. Gomez

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  1. Janice H. Silva
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  2. Renato S. Gomez
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  3. Ana Cristina N. Pinheiro
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Correspondence to Marcus V. Gomez.

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Silva, J.H., Gomez, R.S., Pinheiro, A.C.N. et al. Acetylcholine Release Induced by the Volatile Anesthetic Sevoflurane in Rat Brain Cortical Slices. Cell Mol Neurobiol 25, 807–818 (2005). https://doi.org/10.1007/s10571-005-4934-x

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  • DOI: https://doi.org/10.1007/s10571-005-4934-x

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