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Protective Effects of Adeno-associated Virus Mediated Brain-derived Neurotrophic Factor Expression on Retinal Ganglion Cells in Diabetic Rats

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Abstract

Adeno-associated virus vector plasmid carrying the expression cassette of brain-derived neurotrophic factor (BDNF), pAAV-BDNF, was constructed and packaged into recombinant adeno-associated virus (rAAV-BDNF). The rAAV-BDNF was intravitreally injected into streptzotocin (STZ)-induced diabetic Sprague–Dawley (SD) Rats. Data showed that over-expression of BDNF could increase alive retinal ganglion cell (RGC) number and improve its function in streptzotocin(STZ)-induced diabetic rats, which might be a new method to treat diabetic neuropathy and retinopathy.

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Acknowledgments

We thank Dr. Shigang He and Dr. Ruotong Ren (Institute of biophysics, Chinese academy of sciences) for their help with data collection.

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No potential conflicts of interest.

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Authors and Affiliations

  1. Department of Ophthalmology, Chinese PLA General Hospital, No. 28, Fuxing Road, Haidian District, 100853, Beijing, China

    Yan Gong, Shi-hui Wei, Xiao-fei Chen, Bao-ke Hou, Xin Jin & Mao-nian Zhang

  2. Department of Pathology, Aerospace 731 Hospital, Beijing, China

    Zhan-Ping Chang

  3. Institute of Biophysics, Chinese Academy of Sciences, Beijing, China

    Ruo-Tong Ren

  4. Department of Ophthalmology, The First Affiliated Hospital of General Hospital of PLA, Beijing, China

    Huan-Fen Zhou

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  1. Yan Gong
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  2. Zhan-Ping Chang
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  4. Shi-hui Wei
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  5. Huan-Fen Zhou
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  7. Bao-ke Hou
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  8. Xin Jin
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  9. Mao-nian Zhang
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Correspondence to Mao-nian Zhang.

Additional information

Yan Gong, Zhan-Ping Chang contributed equally to this study.

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Gong, Y., Chang, ZP., Ren, RT. et al. Protective Effects of Adeno-associated Virus Mediated Brain-derived Neurotrophic Factor Expression on Retinal Ganglion Cells in Diabetic Rats. Cell Mol Neurobiol 32, 467–475 (2012). https://doi.org/10.1007/s10571-011-9779-x

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  • DOI: https://doi.org/10.1007/s10571-011-9779-x

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