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Genetic variability and population structure of Grapevine virus A coat protein gene from naturally infected Italian vines

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Abstract

Grapevine virus A (GVA) is considered one of the viruses associated with rugose wood (RW), one of the most economically important diseases of grapevine. Thirty-seven GVA isolates collected from grapevine cultivars from Marche (central-eastern Italy), Apulia and Campania (southern Italy), were subjected to molecular characterization. The genetic and population diversity was studied in the coat protein (CP) gene by RT-PCR-RFLP analysis with three restriction enzymes (MseI, AluI, and AciI), and nucleotide sequencing. A new primer pair (CP1F/R) allowing amplification of the whole CP gene (621 bp) was developed. RFLP with AciI yielded the highest number of variants in GVA isolates, showing seven different ‘simple’ profiles (A, B, C, D, E, F, and G). ‘Complex’ profiles were also found, and the most common variant combination was A + B in 39% of isolates. The analysis of GVA sequences confirmed the presence of plants infected with more than one GVA variant and suggested that RT-PCR-RFLP is suitable for evaluating population diversity of GVA enabling a screening of different haplotypes. The distribution of RFLP profiles and the phylogenetic analysis were not correlated with the location of infected plants, showing the presence of a GVA population with genetic diversity in the average with those of RNA viruses.

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Acknowledgements

The work was supported by the project “Characterization of grapevine viruses in Marche”, funded by Marche Polytechnic University. We are indebted to Drs Pasquale Saldarelli (CNR-IVV, Bari, Italy), Maher Al Rwahnih and Cristina Rosa (UC Davis, Davis, CA, USA) for advice on the interpretation of molecular data. We are grateful to Prof. G.P. Martelli for critical revision of the manuscript.

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Authors and Affiliations

  1. Dipartimento di Scienze Ambientali e delle Produzioni Vegetali, Universitá Politecnica delle Marche, Via Brecce Bianche, Ancona, 60131, Italy

    Sergio Murolo, Gianfranco Romanazzi & Maria Barbara Branzanti

  2. Department of Plant Pathology, University of California, Davis, CA, USA

    Adib Rowhani

  3. Dipartimento di Protezione delle Piante e Microbiologia Applicata, Universitá degli Studi di Bari e Istituto di Virologia Vegetale, CNR Bari, Via Amendola 165/A, 70126, Bari, Italy

    Angelantonio Minafra, Pierfederico La Notte & Vito Savino

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  1. Sergio Murolo
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  2. Gianfranco Romanazzi
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  3. Adib Rowhani
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  4. Angelantonio Minafra
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  5. Pierfederico La Notte
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  6. Maria Barbara Branzanti
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  7. Vito Savino
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Correspondence to Sergio Murolo.

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Murolo, S., Romanazzi, G., Rowhani, A. et al. Genetic variability and population structure of Grapevine virus A coat protein gene from naturally infected Italian vines. Eur J Plant Pathol 120, 137–145 (2008). https://doi.org/10.1007/s10658-007-9202-0

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  • DOI: https://doi.org/10.1007/s10658-007-9202-0

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