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The effect of lipoic acid on wound healing in a full thickness uterine injury model in rats

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Abstract

Aim of this study was to investigate the effects of lipoic acid on uterine wound healing by immunohistochemical and biochemical assay in a rat uterine horn model with full thickness injury. Thirty-two female Wistar albino rats were randomised into five groups: Control group, with no intervention; uterine scar group 15days (US15d), uterine scar group 15 days + alpha lipoic acid (ALA) (US15d + ALA), uterine scar group 30 days (US30d) and uterine scar group 30 days + ALA (US30 days + ALA). After uterine incision 100 mg/kg of ALA was administered by oral gavage for either 15 or 30 days. Vascular endothelial growth factor (VEGF) and alpha smooth muscle actin (α-SMA) distribution were evaluated by immunohistochemical methods in tissue and ELISA methods in tissue homogenate. The percentage of α-SMA positive area in US15d + ALA and US30d + ALA groups was significantly higher than US15 and US30d groups. The percentage of VEGF positive area in US15d + ALA group was significantly higher than US15d group and US30d + ALA group was significantly higher than US30d group. Biochemically, α-SMA was significantly higher in the US15d + ALA group when compared to US15d group and higher in US30d + ALA group when compared to US30d group. VEGF was significantly higher in US15d + ALA and US30d + ALA groups when compared to US15 and US30d groups. In conclusion, ALA was found to be effective in enhancing wound healing in uterine full thickness injury.

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Conflict of interest

All authors have no financial and personal relationships with other people or organizations that could inappropriately influence (bias) their work.

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Authors and Affiliations

  1. Department of Histology and Embryology, Dokuz Eylul University School of Medicine, Izmir, Turkey

    Serap Cilaker Micili, Pinar Akokay & Bekir Uğur Ergur

  2. Department of Obstetrics and Gynecology, Celal Bayar University School of Medicine, Manisa, Turkey

    Asli Goker

  3. Learning Resources Center Research Laboratory, Dokuz Eylul University Medical School, Izmir, Turkey

    Oya Sayin

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  1. Serap Cilaker Micili
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  2. Asli Goker
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  3. Oya Sayin
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  4. Pinar Akokay
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  5. Bekir Uğur Ergur
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Correspondence to Serap Cilaker Micili.

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Micili, S.C., Goker, A., Sayin, O. et al. The effect of lipoic acid on wound healing in a full thickness uterine injury model in rats. J Mol Hist 44, 339–345 (2013). https://doi.org/10.1007/s10735-013-9485-8

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  • DOI: https://doi.org/10.1007/s10735-013-9485-8

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