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Disturbance of intraepithelial lymphocytes in a murine model of acute intestinal ischemia/reperfusion

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Abstract

Strategically located at the epithelial basolateral surface, intraepithelial lymphocytes (IELs) are intimately associated with epithelial cells and maintain the epithelial barrier integrity. Intestinal ischemia–reperfusion (I/R)-induced acute injury not only damages the epithelium but also affects the mucosal barrier function. Therefore, we hypothesized that I/R-induced mucosal damage would affect IEL phenotype and function. Adult C57BL/6J mice were treated with intestinal I/R or sham. Mice were euthanized at 6 h after I/R, and the small bowel was harvested for histological examination and to calculate the transmembrane resistance. Occludin expression and IEL location were detected through immunohistochemistry. The IEL phenotype, activation, and apoptosis were examined using flow cytometry. Cytokine and anti-apoptosis-associated gene expressions were measured through RT-PCR. Intestinal I/R induced the destruction of epithelial cells and intercellular molecules (occludin), resulting in IEL detachment from the epithelium. I/R also significantly increased the CD8αβ, CD4, and TCRαβ IEL subpopulations and significantly changed IEL-derived cytokine expression. Furthermore, I/R enhanced activation and promoted apoptosis in IELs. I/R-induced acute intestinal mucosal damage significantly affected IEL phenotype and function. These findings provide profound insight into potential IEL-mediated epithelial barrier dysfunction after intestinal I/R.

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Acknowledgments

This work was supported through funding from the National Natural Science Foundation of China (NSFC 81020108023 and NSFC 81272078 to H.Y., NSFC 81270451 to W.D.X.) and the Chongqing Science and Technology Commission International Key Collaboration Project (CSTC 201110008 to H.Y.).

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Authors and Affiliations

  1. Department of General Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China

    Yuan Qiu, Min Yu, Yang Yang, Wensheng Wang, Lihua Sun, Guoqing Chen, Yong Liu, Weidong Xiao & Hua Yang

  2. Center of Medical Experiment and Technology, Xinqiao Hospital, Third Military Medical University, Chongqing, China

    Halei Sheng

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  1. Yuan Qiu
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  2. Min Yu
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  3. Yang Yang
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  4. Halei Sheng
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  5. Wensheng Wang
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  6. Lihua Sun
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  7. Guoqing Chen
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  8. Yong Liu
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  9. Weidong Xiao
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  10. Hua Yang
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Correspondence to Hua Yang.

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Yuan Qiu and Min Yu have contributed equally to this study.

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Qiu, Y., Yu, M., Yang, Y. et al. Disturbance of intraepithelial lymphocytes in a murine model of acute intestinal ischemia/reperfusion. J Mol Hist 45, 217–227 (2014). https://doi.org/10.1007/s10735-013-9544-1

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  • DOI: https://doi.org/10.1007/s10735-013-9544-1

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