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IL-17A enhances ADAMTS-7 expression through regulation of TNF-α in human nucleus pulposus cells

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Abstract

ADMATS-7 is known to play an important role in the pathogenesis of various diseases, including cartilaginous diseases. IL-17A is an inflammatory cytokine detected in degenerative disc tissues. However, the interplay between IL-17A and ADMATS-7 in human disc degeneration is still unknown. Samples collected from 50 patients were divided into three groups according to MRI degeneration grading system score. Immunohistochemistry, RT-PCR and western Blotting were used to investigate the expression of ADAMTS-7 in NP tissues. Furthermore, a rat disc degeneration model was established, and the expression level of ADAMTS-7 was assayed using immunohistochemistry, RT-PCR and western Blotting. The human NP cells were cultured in the presence and absence of IL-17A stimulation. RNA extracts were collected, and real-time PCR was performed to determine the expression of ADAMTS-7. Moreover, ADAMTS-7 concentrations were detected in human NP cell culture supernatants by ELISA. After culturing NP cells with IL-17A (with or without Etanercept), ADAMTS-7 levels were detected in each group. ADAMTS-7 expression was dramatically elevated in both human and rat degenerative NP tissues compared with normal controls. The RT-PCR and ELISA results revealed that IL-17A could enhance the production of ADAMTS-7, while ADAMTS-7 expression dramatically decreased in the IL-17A + Etanercept group in comparison to the IL-17A alone group. Our results indicate the presence of ADAMTS-7 in human NP cells and imply its potential role in disc degeneration. Additionally, our results indicate that IL-17A induced ADAMTS-7 expression via TNF-α, which may form a molecular axis in human NP cells.

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Acknowledgments

This study was supported by grants from the Natural Science Foundation of Shandong Province (ZR2013HM095) and the National Natural Science Foundation of China (81572191, 81501880).

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    Authors and Affiliations

    1. Department of Orthopedics, Qilu Hospital of Shandong University, Jinan, 250012, China

      Shuai-Shuai Wang, Wei Zhang, Yuan-Qiang Zhang, Yunpeng Zhao, Yi Liu, Jing-kun Li, Hao-xuan Zhang, Lei Cheng & Lin Nie

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    1. Shuai-Shuai Wang
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    2. Wei Zhang
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    3. Yuan-Qiang Zhang
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    4. Yunpeng Zhao
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    7. Hao-xuan Zhang
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    Correspondence to Lei Cheng or Lin Nie.

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    Shuai–Shuai Wang and Wei Zhang have been contributed equally to this work.

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    Wang, SS., Zhang, W., Zhang, YQ. et al. IL-17A enhances ADAMTS-7 expression through regulation of TNF-α in human nucleus pulposus cells. J Mol Hist 46, 475–483 (2015). https://doi.org/10.1007/s10735-015-9640-5

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    • DOI: https://doi.org/10.1007/s10735-015-9640-5

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