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CD4+, CD25+, FOXP3+ T Regulatory Cell Levels in Obese, Asthmatic, Asthmatic Obese, and Healthy Children

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Abstract

The aim of this prospective case control study is to determine CD4+, CD25+, and FoxP3+ T regulatory cells (Tregs) and T helper cells (Ths) in obese, asthmatic, asthmatic obese, and healthy children. Obese (n = 40), asthmatic (n = 40), asthmatic obese (n = 40), and healthy children (n = 40) were included in this study. Blood samples collected from children were marked with CD4, CD25, ve Foxp3 in order to detect Tregs and Ths by flow cytometric method. Statistical analyses were performed. p ≤ 0.05 was chosen as meaningful threshold. Tregs exhibiting anti-inflammatory nature were significantly lower in obese (0.16 %; p ≤ 0.001), asthmatic (0.25 %; p ≤ 0.01), and asthmatic obese (0.29 %; p ≤ 0.05) groups than control group (0.38 %). Ths were counted higher in asthma group than control (p ≤ 0.01) and obese (p ≤ 0.001) groups. T cell immunity plays important roles in chronic inflammatory diseases such as obesity and asthma pathogeneses. Decreased numbers of Tregs found in obese, asthmatic, and asthmatic obese children might represent a challenge of these cells.

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Abbreviations

APC :

Allophycocyanin

BMI :

Body mass index

FACS :

Fluorescence-activated cell sorting

FITC :

Fluorescein isothiocyanate

GINA :

The Global Initiative for Asthma

PBMCs :

peripheral blood mononuclear cells

PE :

Phycoerythrin

Ths :

Helper T cells

Tregs :

Regulatory T cells

WHO :

World Health Organization

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Acknowledgments

This study was supported by Namik Kemal University Scientific Research Projects Coordination Unit. BAP protocol no: NKUBAP.00.20.AR.12.09/01.06.2012.

Ethical Committee

Protocols have been approved by the Namik Kemal University, Faculty of Medicine, Ethical Committee for Clinical Investigations: NKU protocol no: NKU. 2011.35/30.11.2011.

Conflicts of Interest

None of the authors have reported any conflict of interest related to the present study.

Author information

Authors and Affiliations

  1. Medical Faculty, Department of Pediatrics, Namik Kemal University, Tekirdag, Turkey

    Metin Donma, Burcu Ozdilek & Burcin Nalbantoglu

  2. Ministry of Health, State Hospital, Clinics of Pediatrics, Hakkari, Turkey

    Erkut Karasu

  3. Medical Faculty, Department of Hematology, Namik Kemal University, Tekirdag, Turkey

    Burhan Turgut

  4. Medical Faculty, Department of Biostatistics, Namik Kemal University, Tekirdag, Turkey

    Birol Topcu

  5. Cerrahpasa Medical Faculty, Department of Medical Biochemistry, Istanbul University, Istanbul, Turkey

    Orkide Donma

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  1. Metin Donma
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  2. Erkut Karasu
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Correspondence to Metin Donma.

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Donma, M., Karasu, E., Ozdilek, B. et al. CD4+, CD25+, FOXP3+ T Regulatory Cell Levels in Obese, Asthmatic, Asthmatic Obese, and Healthy Children. Inflammation 38, 1473–1478 (2015). https://doi.org/10.1007/s10753-015-0122-4

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  • DOI: https://doi.org/10.1007/s10753-015-0122-4

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