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Evaluation of 28 marine algae from the Qingdao coast for antioxidative capacity and determination of antioxidant efficiency and total phenolic content of fractions and subfractions derived from Symphyocladia latiuscula (Rhodomelaceae)

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Abstract

The extracts obtained from 28 species of marine algae were evaluated for their antioxidant activity (AA) versus the positive controls butylated hydroxytoluene (BHT), gallic acid (GA), and ascorbic acid (AscA). Most of the tested samples displayed antioxidant activity to various degrees. Among them, the extract of Symphyocladia latiuscula exhibited the strongest AA, which was comparable to BHT, GA, and AscA in radical scavenging activity, as shown in the DPPH (α,α-diphenyl-β-picrylhydrazyl) assay, and higher than those of the positive controls in β-carotene-linoleate assay system. In addition, the ethyl acetate-soluble fraction isolated from the crude extract of S. latiuscula exhibited the highest antioxidant activity in both assay systems. This fraction was further fractionated into seven subfractions (F1-F7) by vacuum liquid chromatography (VLC). F1 and F4 were found to be the most effective subfractions in scavenging DPPH radical assay and in the β-carotene-linoleate assay, respectively. The total phenolic content (TPC) and reducing power (RP) for all of the extracts, fractions, and subfractions (F1–F7) were also determined. The TPC of the 28 extracts ranged from 0.10 to 8.00 gallic acid equivalents (mg/g seaweed dry weight) while the RP ranged from 0.07 to 11.60 ascorbic acid equivalents (mg·g−1 seaweed dry weight). Highly positive relationships between AA and TPC as well as between AA and RP were found for the extracts and fractions, while for the subfractions F1–F7 only weak or no such relations were found. The results obtained from this study indicate that further analysis is needed of those marine algal species that contain the most antioxidant activity in order to identify the active principles.

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Authors and Affiliations

  1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Nanhai Road 7, Qingdao, 266071, People’s Republic of China

    Wei-Wei Zhang, Xiao-Juan Duan, Hai-Lan Huang, Yi Zhang & Bin-Gui Wang

  2. Department of Chemistry, Normal College of Qingdao University, Ningxia Road 308, Qingdao, 266071, People’s Republic of China

    Hai-Lan Huang

  3. Graduate School of the Chinese Academy of Sciences, Beijing, 100039, People’s Republic of China

    Xiao-Juan Duan & Yi Zhang

Authors
  1. Wei-Wei Zhang
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  2. Xiao-Juan Duan
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  3. Hai-Lan Huang
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  4. Yi Zhang
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  5. Bin-Gui Wang
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Correspondence to Bin-Gui Wang.

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Zhang, WW., Duan, XJ., Huang, HL. et al. Evaluation of 28 marine algae from the Qingdao coast for antioxidative capacity and determination of antioxidant efficiency and total phenolic content of fractions and subfractions derived from Symphyocladia latiuscula (Rhodomelaceae). J Appl Phycol 19, 97–108 (2007). https://doi.org/10.1007/s10811-006-9115-x

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  • DOI: https://doi.org/10.1007/s10811-006-9115-x

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