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Biocompatibility of polyhydroxybutyrate microspheres: in vitro and in vivo evaluation

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Abstract

Microspheres have been prepared from the resorbable linear polyester of β-hydroxybutyric acid (polyhydroxybutyrate, PHB) by the solvent evaporation technique and investigated in vitro and in vivo. Biocompatibility of the microspheres has been proved in tests in the culture of mouse fibroblast cell line NIH 3T3 and in experiments on intramuscular implantation of the microspheres to Wistar rats for 3 months. Tissue response to the implantation of polymeric microspheres has been found to consist in a mild inflammatory reaction, pronounced macrophage infiltration that increases over time, involving mono- and poly-nuclear foreign body giant cells that resorb the polymeric matrix. No fibrous capsules were formed around polymeric microparticles; neither necrosis nor any other adverse morphological changes and tissue transformation in response to the implantation of the PHB microparticles were recorded. The results of the study suggest that polyhydroxybutyrate is a good candidate for fabricating prolonged-action drugs in the form of microparticles intended for intramuscular injection.

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Acknowledgments

This study was financially supported by the Russian Ministry of Education and the U.S. Civilian Research & Development Foundation CRDF (Grant No. P1MO002), the Program of the RAS Presidium “Fundamental Research to Medicine” (Project No. 12.5), and the Program of the President of Russia (Grant MK-4149.2006.4).

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Authors and Affiliations

  1. Laboratory of Chemoautotrophic Biosynthesis, Institute of Biophysics SB RAS (Siberian Branch Russian Academy of Sciences), Akademgorodok, 50, Krasnoyarsk, 660036, Russia

    Ekaterina Igorevna Shishatskaya, Olga N. Voinova, Anastasiya V. Goreva & Tatiana G. Volova

  2. Laboratory of Bacterial Bioluminescence, Institute of Biophysics SB RAS (Siberian Branch Russian Academy of Sciences), Akademgorodok, 50, Krasnoyarsk, 660036, Russia

    Olga A. Mogilnaya

  3. Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Svo Bodnyi Av. 79, Krasnoyarsk, 660041, Russia

    Tatiana G. Volova

Authors
  1. Ekaterina Igorevna Shishatskaya
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  2. Olga N. Voinova
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  3. Anastasiya V. Goreva
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  4. Olga A. Mogilnaya
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  5. Tatiana G. Volova
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Correspondence to Ekaterina Igorevna Shishatskaya.

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Shishatskaya, E.I., Voinova, O.N., Goreva, A.V. et al. Biocompatibility of polyhydroxybutyrate microspheres: in vitro and in vivo evaluation. J Mater Sci: Mater Med 19, 2493–2502 (2008). https://doi.org/10.1007/s10856-007-3345-6

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  • DOI: https://doi.org/10.1007/s10856-007-3345-6

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