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Three-dimensional polymer coated 45S5-type bioactive glass scaffolds seeded with human mesenchymal stem cells show bone formation in vivo

  • Tissue Engineering Constructs and Cell Substrates
  • Original Research
  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

45S5-type bioactive glasses are a promising alternative to established substitutes for the treatment of bone defects. Because the three-dimensional (3D) structure of bone substitutes is crucial for bone ingrowth and formation, we evaluated the osteoinductive properties of different polymer coated 3D-45S5 bioactive glass (BG) scaffolds seeded with human mesenchymal stem cells (hMSC) in vivo. BG scaffolds coated with gelatin, cross-linked gelatin, and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) were seeded with hMSC prior to implantation into severe combined immunodeficiency mice. Newly formed bone was evaluated with histomorphometry and micro-computed tomography. Bone formation was detectable in all groups, whereas the gelatin-coated BG scaffolds showed the best results and should be considered in further studies.

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Acknowledgments

The authors thank Tyler Swing for proofreading, Tom Bruckner for the support according the statistical analysis, and Birgit Frey for histomorphometric processing. This study was financed by the research grant of the Center of Orthopedics, Traumatology, and Spinal Cord Injury, Heidelberg University Hospital.

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    Authors and Affiliations

    1. HTRG - Heidelberg Trauma Research Group, Trauma and Reconstructive Surgery, Center of Orthopedics, Traumatology, and Spinal Cord Injury, Heidelberg University Hospital, Schlierbacher Landstraße 200a, 69118, Heidelberg, Germany

      Fabian Westhauser, Matthäus Prokscha, Kai Xiao, Gerhard Schmidmaier & Arash Moghaddam

    2. Clinic of Diagnostic and Interventional Radiology (DIR), Heidelberg University Hospital, Im Neuenheimer Feld 110, 69120, Heidelberg, Germany

      Christian Weis & Hans-Ulrich Kauczor

    3. Clinic for Hand-, Plastic- and Reconstructive Surgery, BG-Unfallklinik Ludwigshafen, Ludwig-Guttmann-Straße 13, 67071, Ludwigshafen am Rhein, Germany

      Leonie A. Bittrich & Ulrich Kneser

    4. Institute of Biomaterials, University of Erlangen-Nuremberg, Cauerstr. 6, 91058, Erlangen, Germany

      Wei Li & Aldo R. Boccaccini

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    1. Fabian Westhauser
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    2. Christian Weis
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    5. Wei Li
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    11. Arash Moghaddam
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    Correspondence to Arash Moghaddam.

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    Fabian Westhauser and Christian Weis have contributed equally to this work.

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    Westhauser, F., Weis, C., Prokscha, M. et al. Three-dimensional polymer coated 45S5-type bioactive glass scaffolds seeded with human mesenchymal stem cells show bone formation in vivo. J Mater Sci: Mater Med 27, 119 (2016). https://doi.org/10.1007/s10856-016-5732-3

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    • DOI: https://doi.org/10.1007/s10856-016-5732-3

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