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Influence of common preanalytical variations on the metabolic profile of serum samples in biobanks

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Abstract

A blood pre-centrifugation delay of 24 h at room temperature influenced the proton NMR spectroscopic profiles of human serum. A blood pre-centrifugation delay of 24 h at 4°C did not influence the spectroscopic profile as compared with 4 h delays at either room temperature or 4°C. Five or ten serum freeze–thaw cycles also influenced the proton NMR spectroscopic profiles. Certain common in vitro preanalytical variations occurring in biobanks may impact the metabolic profile of human serum.

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Abbreviations

SST:

Serum separation tube

SPREC:

Standard preanalytical code

TSP:

3-(trimethylsilyl) 3,3,3,3-tetradeutero-propionic acid

CPMG:

Carr-Purcell-Meiboom-Gill

HSQC:

Heteronuclear single quantum coherence

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Acknowledgments

This work was supported by the Conseil Régional de Picardie. We are grateful to Brian de Witt and Karsten Hiller for critical reading of the manuscript.

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Authors and Affiliations

  1. Laboratoire de Phytotechnologie EA 3900-BioPI, University of Picardie Jules Verne, Amiens cedex 1, 80037, Amiens, France

    Ophélie Fliniaux, Antoine Lion & François Mesnard

  2. Biobanque de Picardie, Avenue René Laënnec, 80480, Salouel, France

    Gwenaelle Gaillard

  3. Plateforme Analytique, Bâtiment Serres-Transfert, rue de Mai/rue Dallery, 80039, Amiens cedex 1, France

    Dominique Cailleu

  4. Integrated Biobank of Luxembourg, 6 rue Ernest Barble, 1210, Luxembourg, Luxembourg

    Fotini Betsou

Authors
  1. Ophélie Fliniaux
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  2. Gwenaelle Gaillard
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  3. Antoine Lion
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  4. Dominique Cailleu
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  5. François Mesnard
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  6. Fotini Betsou
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Correspondence to François Mesnard.

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Fliniaux, O., Gaillard, G., Lion, A. et al. Influence of common preanalytical variations on the metabolic profile of serum samples in biobanks. J Biomol NMR 51, 457–465 (2011). https://doi.org/10.1007/s10858-011-9574-5

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  • DOI: https://doi.org/10.1007/s10858-011-9574-5

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