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Peak Multiphoton Excitation of mCherry Using an Optical Parametric Oscillator (OPO)

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Abstract

mCherry is a red fluorescent protein which is bright, photostable, and has a low molecular weight. It is an attractive choice for multiphoton fluorescence imaging; however, the multiphoton excitation spectrum of mCherry is not known. In this paper we report the two photon excitation spectrum of mCherry measured up to 1190 nm in the near infrared (NIR) region. Skin tissues of transgenic mice that express mCherry were used in the experiments. mCherry in the tissues was excited with a Titanium:Sapphire laser and an optical parametric oscillator pumped by the Titanium:Sapphire laser. We found that the peak excitation of mCherry occurs at 1160 nm.

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Acknowledgement

This work was supported by the RO1 grant (R01 HL 077187), Quantum grant (NIH-NIBIB P20 EB007076), NIH training grant (T32 HL007676), and a training fellowship from the Nanobiology Training Program of the Keck Center of the Gulf Coast Consortia (R90DK071504). The authors would like to thank Marco Arrigoni (Coherent Inc., California, USA) for sharing the OPO and Darren Chomiak (Coherent Inc., California, USA) for helpful discussions.

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Authors and Affiliations

  1. Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX, 77030, USA

    Tegy J. Vadakkan

  2. Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX, 77030, USA

    James C. Culver

  3. Department of Bioengineering, Rice University, Houston, TX, 77005, USA

    Liang Gao

  4. Carl Zeiss MicroImaging GmbH, Carl-Zeiss-Promenade 10, 07745, Jena, Germany

    Tiemo Anhut

  5. Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX, 77030, USA

    Mary E. Dickinson

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  1. Tegy J. Vadakkan
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  2. James C. Culver
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  3. Liang Gao
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  4. Tiemo Anhut
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  5. Mary E. Dickinson
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Correspondence to Tegy J. Vadakkan.

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Vadakkan, T., Culver, J., Gao, L. et al. Peak Multiphoton Excitation of mCherry Using an Optical Parametric Oscillator (OPO). J Fluoresc 19, 1103–1109 (2009). https://doi.org/10.1007/s10895-009-0510-y

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  • DOI: https://doi.org/10.1007/s10895-009-0510-y

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