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Optical Properties of Methyl Orange-Doped Droplet and Photodynamic Therapy of Staphylococcus aureus

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Abstract

Dye-doped droplets are known as mixtures of dyes with uniform solutions of water droplets in a continuous phase of oils with surfactants. To observe the relationship between water droplet structures and surfactant types on optical properties of dyes, a mixture of methyl orange (MO)-doped droplet prepared with benzane and hexane as oils and sodium bis(2-ethylhexyl) sulfosuccinate (AOT) as a surfactant was thus examined using Z-scan instrument, spectrophotometer, and fluorimeter in the present study. The findings revealed that nonlinear refractive (NLR) index, nonlinear absorption (NLA) coefficient, as well as fluorescence intensity of the MO had enhanced following a reduction in the droplet water content induced by changes in the non-centrosymmetric charge density distribution of this pH indicator. Moreover, the MO-doped droplet in a continuous phase of benzene investigated by 1H nuclear magnetic resonance (NMR) spectroscopy indicated that the MO had been located in the droplet in the vicinity of the hydrophilic part of the surfactant. Furthermore, the MO-doped droplets along with laser radiation were employed to perform antibacterial photodynamic therapy (APDT) of Staphylococcus aureus (S. aureus). It was ultimately concluded that the bacteria colony had also extremely diminished in the group treated by the MO-doped droplet.

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Acknowledgments

Thanks of Ferdowsi University of Mashhad for Project no: 2/49956. This work was supported by the research grant of Kazan Federal University.

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Authors and Affiliations

  1. Department of Physics, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

    Soheil Sharifi & Afshin Azarpour

  2. Kazan (Volga Region) Federal University, 18 Kremlevskaya St., Kazan, Russian Federation, 420008

    Shurshalova Guzel Salavatovna

  3. Department of Pediatrics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Forough Rakhshanizadeh

  4. Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, PO Box 91775, Mashhad, Iran

    Gholamhossein Zohuri

  5. Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran

    Mohammad Reza Sharifmoghadam

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  1. Soheil Sharifi
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Sharifi, S., Salavatovna, S.G., Azarpour, A. et al. Optical Properties of Methyl Orange-Doped Droplet and Photodynamic Therapy of Staphylococcus aureus. J Fluoresc 29, 1331–1341 (2019). https://doi.org/10.1007/s10895-019-02459-0

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