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DNA damage induces N-acetyltransferase NAT10 gene expression through transcriptional activation

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Abstract

NAT10 (N-acetyltransferase 10) is a protein with histone acetylation activity and primarily identified to be involved in regulation of telomerase activity. The presented research shows its transcriptional activation by genotoxic agents and possible role in DNA damage. NAT10 mRNA could be markedly increased by using hydrogen peroxide (H2O2) or cisplatin in a dose- and time-dependent way, and the immunofluorescent staining revealed that the treatment of H2O2 or cisplatin induced focal accumulation of NAT10 protein in cellular nuclei. Both H2O2 and cisplatin could stimulate the transcriptional activity of the NAT10 promoter through the upstream sequences from −615 bp to +110 bp, with which some nuclear proteins interacted. Ectopic expression of NAT10 could enhance the number of survival cells in the presence of H2O2 or cisplatin. The above results suggested that NAT10 could be involved in DNA damage response and increased cellular resistance to genotoxicity.

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (grant No. 30570691), Doctors Program of Chinese Education Ministry (20040001147), and a grant from the center of human genetic disease, Peking University. We thank Dr. Jan SjÖberg for editing the manuscript in English.

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  1. Department of Pathology, Health Science Center of Peking University, 38 Road Xueyuan, Haidian District, Beijing, 100083, China

    Haijing Liu, Yun Ling, Yilei Gong, Ying Sun, Lin Hou & Bo Zhang

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  1. Haijing Liu
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  2. Yun Ling
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  3. Yilei Gong
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  4. Ying Sun
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  5. Lin Hou
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Correspondence to Bo Zhang.

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Liu, H., Ling, Y., Gong, Y. et al. DNA damage induces N-acetyltransferase NAT10 gene expression through transcriptional activation. Mol Cell Biochem 300, 249–258 (2007). https://doi.org/10.1007/s11010-006-9390-5

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  • DOI: https://doi.org/10.1007/s11010-006-9390-5

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