Abstract
We aimed to examine the nucleoside triphosphate diphosphohydrolases (NTPDase) in lymphocytes; adenosine deaminase (ADA) and butyrylcholinesterase (BChE) in serum; and acetylcholinesterase (AChE), superoxide dismutase (SOD), and catalase (CAT) activity in whole blood; since these enzymes are involved in inflammation responses as well as in oxidative stress conditions. We also checked the levels of total thiols (T-SH), non-protein thiols (NPSH), and thiobarbituric acid reactive substances (TBARS) in serum of patients with lung cancer. We collected blood samples from patients (n = 31) previously treated for lung cancer with chemotherapy. Patients were classified as stage IIIb and IV according to the Union for International Cancer Control (UICC). The results showed a significant increase in the hydrolysis of ATP, ADP, and adenosine in patients when compared with the control group. The activity of AChE, SOD, and CAT as well as the T-SH and NPSH levels were higher in patients group and TBARS levels were lower in patients compared with the control group. These findings demonstrated that the enzymes activity involved in the control of inflammatory and immune processes as well as the oxidative stress parameters are altered in patients with lung cancer.
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Acknowledgments
The authors wish to thank all the lung cancer patients and the professionals at the Hematology/Oncology Laboratory (HUSM) for their support. This study was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Rio Grande do Sul (FAPERGS), Fundação Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Instituto Brasileiro de Neurociência (IBN-Net), INCT for Excitotoxicity and Neuroprotection, and the Federal University of Santa Maria, RS, Brazil.
Disclosures
This work is original and is not under consideration by another journal. All the patients signed the written consent and the work was approved by the Human Ethical Committee from the Federal University of Santa Maria Hospital. Finally, this manuscript has been approved by all authors and has no conflict of interest.
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Zanini, D., Schmatz, R., Pelinson, L.P. et al. Ectoenzymes and cholinesterase activity and biomarkers of oxidative stress in patients with lung cancer. Mol Cell Biochem 374, 137–148 (2013). https://doi.org/10.1007/s11010-012-1513-6
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DOI: https://doi.org/10.1007/s11010-012-1513-6