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RETRACTED ARTICLE: MiRNA-26b inhibits the proliferation, migration, and epithelial–mesenchymal transition of lens epithelial cells

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This article was retracted on 24 April 2024

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Abstract

MicroRNAs (miRNAs) are a class of small endogenous gene regulators that play important roles in various developmental and pathological processes. However, little is known about the precise identity and functions of miR-26b in posterior capsule opacification (PCO). In this study, we report that the expression of miR-26b is decreased in human PCO-attached lens epithelial cells (LECs) and SRA01/04 cells in the presence of TGF-β2. Overexpression of miR-26b inhibited the proliferation of LECs based on MTT assays and BrdU incorporation assays. In addition, the overexpression of miR-26b inhibited the migration ability of LECs, as shown by wound-healing and transwell migration assays. The overexpression of miR-26b increased the level of the lens epithelial marker E-cadherin and reduced the levels of mesenchymal-related proteins, such as fibronectin, a-SMA, and type I collagen, in SRA01/04 cells in the presence of TGF-β2. Furthermore, the upregulation of E-cadherin and downregulation of mesenchymal-related proteins were induced in human PCO-attached LECs transfected with miR-26b mimics. We further demonstrated that Smad4 and COX-2 are targets of miR-26b in LECs using luciferase reporter assays. These data reveal that miR-26b can inhibit the proliferation, migration, and EMT of lens epithelial cells, and restoration of miRNA-26b may be a potential, novel therapeutic target for the prevention and treatment of posterior capsule opacification.

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Acknowledgments

This work was supported by research Grants from the National Natural Science Foundation for the Young Scholars of Beijing Shijitan Hospital, Capital Medical University (No. 2013-QB01), and the National Natural Science Foundation of China (No. 81270984).

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Authors and Affiliations

  1. Department of Ophthalmology, Beijing Shijitan Hospital, Capital Medical University, Beijing, People’s Republic of China

    Ning Dong & Bing Xu

  2. Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA

    Silvia R. Benya

  3. Tianjin Eye Hospital, Tianjin Medical University, 4, Gansu Road, Heping District, Tianjin, 300020, China

    Xin Tang

Authors
  1. Ning Dong
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  2. Bing Xu
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  3. Silvia R. Benya
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  4. Xin Tang
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Corresponding author

Correspondence to Xin Tang.

Additional information

Ning Dong and Bing Xu have contributed equally to this work.

This article has been retracted. Please see the retraction notice for more detail: https://doi.org/10.1007/s11010-024-05017-w

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Dong, N., Xu, B., Benya, S.R. et al. RETRACTED ARTICLE: MiRNA-26b inhibits the proliferation, migration, and epithelial–mesenchymal transition of lens epithelial cells. Mol Cell Biochem 396, 229–238 (2014). https://doi.org/10.1007/s11010-014-2158-4

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  • DOI: https://doi.org/10.1007/s11010-014-2158-4

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