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MicroRNA-132-3p represses Smad5 in MC3T3-E1 osteoblastic cells under cyclic tensile stress

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Abstract

MicroRNAs (miRNAs) regulate osteogenic differentiation of bone cells, which has applications in orthodontics. Here we evaluated the miRNA expression profile of MC3T3-E1 osteoblasts under cyclic tensile stress with chip technology and found that miR-132-3p was up-regulated by 12% cyclic tensile stress. Alkaline phosphatase activity and osteocalcin expression in MC3T3-E1 cells were decreased under these conditions. Smad2 and Smad5 were identified as potential target genes of miR-132-3p. Native and phosphorylated Smad2 and Smad5 expression was negatively correlated with miR-132-3p levels in the cells under cyclic stretch; however, only Smad5 protein level was reduced upon miR-132-3p overexpression. The luciferase reporter assay confirmed a direct interaction between miR-132-3p and Smad5. Thus, miR-132-3p maybe regulates osteoblast differentiation via Smad5 in response to cyclic tensile stress.

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Acknowledgements

This work was supported by the natural science foundation of China (81400566, 81302220), the natural science foundation of Shanxi Province (2014011027-2, 2014021037-1), the Doctoral Startup Research Fund of Shanxi Medical University (B03201204), and sponsored by the Fund for Shanxi “1331 Project” Key Subjects Construction. We would like to thank Editage http://www.editage.cn for English language editing.

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  1. MingYan Liu and Fen Sun contributed equally to this work.

Authors and Affiliations

  1. Department of Orthodontics, Dental Hospital, Shanxi Medical University, Taiyuan, China

    MingYan Liu, Fen Sun & YunXia Feng

  2. Suzhou Dental Doctor Outpatient Department Co. LTD, Suzhou, China

    MingYan Liu

  3. School of Basic Medical Science, Shanxi Medical University, 56 Xinjian Road, Ying Ze, Taiyuan, 030001, China

    XinYi Sun & Ming Liu

  4. Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, 15 Changle Xi Road, Xi’an, China

    Juan Li

  5. Aoji Bio-Tech Co., Ltd, Shanghai, China

    Qiang Fan

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  1. MingYan Liu
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Liu, M., Sun, F., Feng, Y. et al. MicroRNA-132-3p represses Smad5 in MC3T3-E1 osteoblastic cells under cyclic tensile stress. Mol Cell Biochem 458, 143–157 (2019). https://doi.org/10.1007/s11010-019-03538-3

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