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Molecular characterization of the sheep CIB1 gene

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Abstract

The calcium and integrin binding protein 1(CIB1), is an EF-hand-containing protein that binds many effector proteins including the platelet αIIbβ3 integrin and potentially regulates their functions. Here we report the cloning and characterization of the sheep CIB1 gene. The CIB1 cDNA is 885-bp in size, containing a 45-bp of 5′ untranslated region (UTR), a 264-bp long 3′-UTR and a 576-bp open reading frame that encodes 191 amino acids. The sheep CIB1 cDNA shows 98.3, 92.0, 91.8, 91.3, 90.5 and 90.1% of similarity, at the nucleotide level, to its equivalents in cattle, pigs, rhesus monkey, humans, rats and mice, respectively at the deduced protein level, the corresponding values are more than 94%. The sheep CIB1 gene consisted of seven exons. Quantitative PCR (Q-PCR) showed that CIB1 was widely expressed in different tissues with the highest level in the testis, suggesting that it may play a role in ram fertility. We cloned the sheep CIB2, CIB3 and CIB4 genes and detected their expression patterns in different tissues.

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Acknowledgments

This work was supported by the 863 Hi-Tech Research and Development Program of China (2006AA10Z199) and the National Science and Technology Pillar Program (Project No. 2006BAD01A11). We special thank to Dr. Yaofeng Zhao for critical reading of this manuscript. We are also indebted to staffs of the National Center for Molecular Genetics and Breeding of Animal for sample collection.

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Authors and Affiliations

  1. College of Animal Science and Technology, China Agricultural University, 100094, Beijing, People’s Republic of China

    Yan Yu & Chuduan Wang

  2. Institute of Animal Science, Zhejiang Academy of Agricultural Sciences, 310021, Hangzhou, People’s Republic of China

    Xuemei Song

  3. Institute of Animal Science, Chinese Academy of Agricultural Sciences, 100094, Beijing, People’s Republic of China

    Lixin Du

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  1. Yan Yu
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Correspondence to Chuduan Wang.

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Yu, Y., Song, X., Du, L. et al. Molecular characterization of the sheep CIB1 gene. Mol Biol Rep 36, 1799–1809 (2009). https://doi.org/10.1007/s11033-008-9383-4

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