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Effects of propofol on pro-inflammatory cytokines and nuclear factor kappaB during polymicrobial sepsis in rats

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Abstract

Nuclear factor kappa B (NF-κB) plays a central role in regulating the transcription of several genes associated with sepsis/septic shock. Therefore, the author investigated the effects of propofol on the plasma tumor necrosis factor α and interleukin 6 (TNF-α and IL-6) levels and NF-κB activation during polymicrobial sepsis in rats. Male Sprague-Dawlay rats were subjected to cecal ligation and puncture (CLP, a model of polymicrobial sepsis) or sham operation. The animals were randomly assigned into four equal groups (n = 10): sham CLP group, CLP group, PPF (propofol) I group and PPF II group. Thirty minutes before CLP, propofol (5 and 10 mg kg−1 h−1, respectively) was infused continuously through the left femoral vein cannula in PPF I group or PPF II group, CLP group and sham CLP group receiving 0.9% saline only at the rates of 5 ml kg−1 h−1. The right femoral artery was cannulated to monitor mean arterial pressure (MAP) and heart rates (HR). CLP produced progressive hypotension and a first increase followed by a decrease in HR. The plasma TNF-α and IL-6 levels and the hepatic NF-κB activation significantly increased after CLP alone. Compared with CLP group, propofol treatment reversed hypotension, slightly steadied heartbeats, and decreased the plasma TNF-α and IL-6 levels, and significantly suppressed NF-κB activation. Propofol has inhibited the hepatic NF-κB activition and the pro-inflammatory cytokine response during polymicrobial sepsis in rats.

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Acknowledgments

This study was supported in part by the Natural Science Foundation of Hubei Province of China (No. 2002AB147). Institutional Animal Care and Use Committee, Wuhan University approved all the animal studies.

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Authors and Affiliations

  1. The Research Centre of Anesthesiology and Critical Care Medicine, Zhongnan Hospital, Wuhan University, 430071, Wuhan, Hubei Province, China

    Xue-Min Song, Yan-Lin Wang, Jian-Guo Li, Cheng-Yao Wang, Qing Zhou, Zong-Ze Zhang & Hui Liang

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  1. Xue-Min Song
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  2. Yan-Lin Wang
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Correspondence to Yan-Lin Wang.

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Song, XM., Wang, YL., Li, JG. et al. Effects of propofol on pro-inflammatory cytokines and nuclear factor kappaB during polymicrobial sepsis in rats. Mol Biol Rep 36, 2345–2351 (2009). https://doi.org/10.1007/s11033-009-9456-z

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  • DOI: https://doi.org/10.1007/s11033-009-9456-z

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