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Connexin43 interacts with Caveolin-3 in the heart

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Abstract

Gap junctions (GJs), collections of multiple intercellular channels between neighboring cells, are specialized channels facilitating intercellular electrical and chemical communication. GJs are important for synchronizing coupling and coordinated contraction in the heart, and are crucial regulators of heart gene transcription, cardiac development, and protection of ischemic cardiomyocytes through second messenger communication. Identification of proteins that interact with Connexin43 (Cx43), the predominant protein in cardiac GJs, may contribute to the understanding of GJ functional regulation. Using a yeast two-hybrid system, we identified Caveolin-3 (Cav3) as a new Cx43-interacting protein. This interaction was confirmed by co-immunoprecipitation and co-localization experiments. CX43 interacts with Cav3, suggesting that Cav3 may participate in the functional regulation of GJs.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (project number 30600254). We gratefully acknowledge the assistance of Dr. Yucai Fu, Laboratory of Cell Senescence, Shantou University Medical College, for helpful suggestions and assistance.

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Authors and Affiliations

  1. Department of Cardiology, The First Affiliated Hospital, Shantou University Medical College, 57 Changping Road, 515041, Shantou, People’s Republic of China

    Limei Liu, Yuguang Li, Jijin Lin, Qing Liang, Xiuzhang Sheng, Jun Wu, Ruiyan Huang, Shukai Liu & Yanyan Li

  2. Department of Cardiology, Guangdong Provincial People’s Hospital, Guangdong Provincial Cardiovascular Institute, 510080, Guangzhou, China

    Jijin Lin

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  1. Limei Liu
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  2. Yuguang Li
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  3. Jijin Lin
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Correspondence to Yuguang Li.

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Liu, L., Li, Y., Lin, J. et al. Connexin43 interacts with Caveolin-3 in the heart. Mol Biol Rep 37, 1685–1691 (2010). https://doi.org/10.1007/s11033-009-9584-5

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  • DOI: https://doi.org/10.1007/s11033-009-9584-5

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