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Dominant-positive HSF1 decreases alpha-synuclein level and alpha-synuclein-induced toxicity

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Abstract

Alpha-synuclein aggregation and cytotoxicity are widely considered to play a critical role in the process of Parkinson’s disease. Heat shock proteins are a large family of cellular protective molecules in most kinds of cells. In this study, we examined the impact of dominant-positive heat shock transcription factor 1 (HSF1) on alpha-synuclein over-expression cellular model of Parkinson’s disease. We found that over-expression of alpha-synuclein could form alpha-synuclein immunopositive inclusions and result in cell death; dominant-positive HSF1 dramatically increased the expression of HSP70 in SH-SY5Y cells, and significantly decreased the level and cytotoxicity of alpha-synuclein. Taken together, these data indicate that dominant-positive HSF1 plays an important role in suppressing alpha-synuclein aggregation and toxicity in SH-SY5Y cells. Parkinson’s disease which is marked by alpha-synuclein aggregation may be treated by increasing a set of endogenous heat shock proteins.

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Acknowledgments

The research was supported by the grants from Guangdong Natural Science Foundation (6026372) and Guangdong Institute of Chinese Medicine (1060165). We sincerely thank Dr. Richard Voellmy for his generous gift of the plasmid pcDNA-HSF1(+).

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Authors and Affiliations

  1. School of Life Sciences, University of Science and Technology of China, 230027, Hefei, China

    Xu Liangliang

  2. Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, 510663, Guangzhou, China

    Xu Liangliang, Hou Yonghui, E. Shunmei, Gong Shoufang, Zhou Wei & Zou Jiangying

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  1. Xu Liangliang
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  2. Hou Yonghui
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  3. E. Shunmei
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Correspondence to Zou Jiangying.

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Liangliang, X., Yonghui, H., Shunmei, E. et al. Dominant-positive HSF1 decreases alpha-synuclein level and alpha-synuclein-induced toxicity. Mol Biol Rep 37, 1875–1881 (2010). https://doi.org/10.1007/s11033-009-9623-2

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  • DOI: https://doi.org/10.1007/s11033-009-9623-2

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