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Effects of ROCK inhibitor, Y-27632, on adhesion and mobility in esophageal squamous cell cancer cells

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Abstract

Rho-associated protein kinase (ROCK), a molecular switch, modulates cellular functions in many cancers, such as hepatocellular, breast, colon cancers, etc. However, little is known the effect of ROCK on cell adhesion and mobility in esophageal squamous cell cancer (ESCC), one of the most diagnosed cancers in China. In this study, Y-27632 was used to specifically block ROCK activity in ESCC cells. Adhesion of ESCC cells was detected by homotypic and heterotypic adhesion assay together with examination of E-cadherin expression. Motility of ESCC cells changes were examined by detection of phosphorylated cofilin and observed under confocal microscopy, respectively. We found that Y-27632 increased both heterotypic and homotypic adhesion, and the expression of E-cadherin; decreased phosphorylated cofilin resulting in actin rearrangement in ESCC cells. All these findings indicate that ROCK signaling pathway plays an important role in cell adhesion and mobility, suggesting that it may be used as a potential target for therapy of ESCC.

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Acknowledgments

This study was supported by the grants from Key Programs of Science and Technology of Education Ministry of China (No. 00190) and the Tenth Five-Year Plan’s “211 Projects “of Education Ministry of China (No. 2002-2).

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Authors and Affiliations

  1. Institute of Tumor Molecular Surgery, The First Affiliated Hospital, Zhengzhou University Medical College, 40 Daxue Road, 450052, Zhengzhou, Henan, China

    Lili Wang & Lexun Xue

  2. Laboratory for Cell Biology, Department of Biology, Zhengzhou University, 100 Kexue Avenue, 450001, Zhengzhou, China

    Lexun Xue, Hongxia Yan, Jie Li & Yucheng Lu

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  1. Lili Wang
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  2. Lexun Xue
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  3. Hongxia Yan
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  5. Yucheng Lu
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Correspondence to Lexun Xue.

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Wang, L., Xue, L., Yan, H. et al. Effects of ROCK inhibitor, Y-27632, on adhesion and mobility in esophageal squamous cell cancer cells. Mol Biol Rep 37, 1971–1977 (2010). https://doi.org/10.1007/s11033-009-9645-9

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  • DOI: https://doi.org/10.1007/s11033-009-9645-9

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