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HLA association with nasopharyngeal carcinoma in southern Tunisia

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Abstract

In order to study the association of HLA-A, -B and/or DRB1, DQB1 and the nasopharyngeal carcinoma (NPC), 141 patients affected with NPC were typed for the HLA class I by serology method of microlymphocytotoxicity. Among these patients 101 were genotyped for HLA class II system by the PCR-SSP technique. HLA typing results were compared to those of 116 controls. We found that the HLA-A31 and -A33 antigens were significantly more expressed in patients than in the controls (P = 0.016 and 0.010, respectively) and the HLA-A19 antigen, was significantly more frequent in patients when compared to the controls (P = 0.007). The HLA-DRB1*03 and DRB1*13 alleles were significantly more frequent in patients as compared to the controls. The DRB1*01 allele was expressed with a frequency of 20.69% in the controls whereas it was only detected in 3.96% of the NPC patients. Furthermore, the DQB1*05 allele was expressed at a frequency which was significantly less important in affected patient (P = 0.03), whereas, the DQB1*02 allele was more frequent in patients (P = 0.643 × 10−4). Thus our study revealed a significant increase of HLA-A31, A33, A19, B16, B53 and DRB1*03, DRB1*13 and DQB1*02 alleles in our patients. These markers could play a predisposing role in the development of NPC. In contrast, a decrease of HLA-B14, -B35 and DRB1*01 and DQB1*05 alleles was found suggesting a likely protective effect.

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Acknowledgments

In this study, financial support was provided by NPC Research Unit in the University of Medicine of Sfax.

Conflict of interest statement

There is no conflict of interest related to this study.

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Authors and Affiliations

  1. Immunology Laboratory, University Hospital Sfax, Sfax, Tunisia

    Hafedh Makni & Nedia Mahfoudh

  2. Radiotherapy Department, University Hospital Sfax, Sfax, Tunisia

    Jamel Daoud, Hanène Ben Salah & Olfa Haddar

  3. Department of Virology, University Hospital Sfax, Sfax, Tunisia

    Héla Karray

  4. Department of Pathology, University Hospital Sfax, Sfax, Tunisia

    Tahya Boudawara & Abdelmajid Khabir

  5. E.N.T Department, University Hospital Sfax, Sfax, Tunisia

    Abdelmonême Ghorbel

  6. Medical Oncology Department, University Hospital Sfax, Sfax, Tunisia

    Mounir Frikha

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  1. Hafedh Makni
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  2. Jamel Daoud
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  3. Hanène Ben Salah
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  4. Nedia Mahfoudh
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  9. Abdelmajid Khabir
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Correspondence to Abdelmajid Khabir.

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Makni, H., Daoud, J., Ben Salah, H. et al. HLA association with nasopharyngeal carcinoma in southern Tunisia. Mol Biol Rep 37, 2533–2539 (2010). https://doi.org/10.1007/s11033-009-9769-y

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  • DOI: https://doi.org/10.1007/s11033-009-9769-y

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