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Molecular characterization and expression patterns of Lbx1 in porcine skeletal muscle

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Abstract

Ladybird-like genes were recently identified in mammals. The first member characterized, Lbx1, is expressed in developing skeletal muscle and the nervous system. However, little is known about the porcine Lbx1 gene. In the present study, we cloned and characterized Lbx1 from porcine muscle. RT-PCR analyses showed that Lbx1 was highly expressed in porcine skeletal muscle tissues. And we provide the first evidence that Lbx1 has a certain regulated expression pattern during the postnatal period of the porcine skeletal muscle development. Lbx1 gene expressed at higher levels in biceps femoris muscles compared with masseter, semitendinosus and longissimus dorsi muscles in Meishan pigs. Phylogenetic tree was constructed by aligning the amino acid sequences of different species. Moreover, single nucleotide polymorphism (SNP) scanning in the Lbx1 genomic fragment identified two mutations, g.752A>G and g.−1559C>G. Association analysis in our experimental pig populations showed that the mutation of g.752A>G was significantly associated with loin muscle area (P < 0.05) and internal fat rate (P < 0.05). Our results suggest that the Lbx1 gene might be a candidate gene of carcass traits and provide useful information for further studies on its roles in porcine skeletal muscle.

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Acknowledgments

This study was supported financially by the National “863” project of P. R. China (2007AA10Z166), National High Technology Development Project (2006BAD01A08-01), and Hubei Province Key Project of Science and Technology (2006AA202A01).

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Authors and Affiliations

  1. Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College of Animal Science, Huazhong Agricultural University, Wuhan, Hubei, 430070, People’s Republic of China

    Zhe Chao, Jian Wu, Rong Zheng, Feng-E. Li, Yuan-Zhu Xiong & Chang-Yan Deng

  2. Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science, Huazhong Agricultural University, Wuhan, Hubei, 430070, People’s Republic of China

    Zhe Chao, Jian Wu, Rong Zheng, Feng-E. Li, Yuan-Zhu Xiong & Chang-Yan Deng

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  1. Zhe Chao
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  2. Jian Wu
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  3. Rong Zheng
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  4. Feng-E. Li
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  5. Yuan-Zhu Xiong
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  6. Chang-Yan Deng
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Correspondence to Chang-Yan Deng.

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Chao, Z., Wu, J., Zheng, R. et al. Molecular characterization and expression patterns of Lbx1 in porcine skeletal muscle. Mol Biol Rep 38, 3983–3991 (2011). https://doi.org/10.1007/s11033-010-0516-1

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  • DOI: https://doi.org/10.1007/s11033-010-0516-1

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