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Matrix metalloproteinas-9 functional promoter polymorphism 1562C>T increased risk of early-onset coronary artery disease

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Abstract

The Matrix metalloproteinas-9 functional promoter polymorphism 1562C>T may be considered an important genetic determinant of early-onset coronary artery disease (ECAD). In this study, association between MMP-9 1562C>T allele with plasma MMP-9 activity, homocysteine and lipid–lipoproteins level and ECAD in Iranian subjects was investigated. This case–control study consisted of 53 ECAD patients (age < 55 years) and unrelated late-onsets CAD (age > 70 years) who angiographically had at least 50% stenosis. MMP-9 1562C>T polymorphism was detected by PCRRFLP, plasma MMP-9 activity, serum lipid and homocysteine levels were determined by gelatin gel zymography, enzyme assay and by HPLC, respectively. The presence of MMP-9 1562C>T allele was found to be associated with ECAD (OR = 3.2, P = 0.001). The ECAD patients with MMP-9 1562C>T allele had higher MMP-9 activity (P = 0.001), LDL-C (P = 0.045), TC (P = 0.02) and homocysteine (P = 0.01) levels than the LCAD subjects. MMP-9 1562C>T allele is a risk factor for ECAD. The carriers of this allele have high levels of MMP-9 activity, LDL-C, TC and homocysteine (P = 0.01), thus, are more likely to develop myocardial infarction and CAD at young age (less than 55 years).

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Acknowledgment

This research was supported by Tehran University of Medical Sciences and Health Services. Grant no. 18898.

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Authors and Affiliations

  1. Department of Clinical Biochemistry, Tehran University of Medical Sciences, Tehran, Iran

    Massoud Saedi, Asad Vaisi-Raygani, Shahnaz Khaghani, Ahmad Shariftabrizi, M. Rezaie, Parvin Pasalar & Tayebeh Pourmotabbed

  2. Fertility Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Asad Vaisi-Raygani

  3. Department of Clinical Biochemistry, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Asad Vaisi-Raygani & Zohreh Rahimi

  4. Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, TN, USA

    Tayebeh Pourmotabbed

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  1. Massoud Saedi
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Correspondence to Asad Vaisi-Raygani.

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Saedi, M., Vaisi-Raygani, A., Khaghani, S. et al. Matrix metalloproteinas-9 functional promoter polymorphism 1562C>T increased risk of early-onset coronary artery disease. Mol Biol Rep 39, 555–562 (2012). https://doi.org/10.1007/s11033-011-0770-x

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  • DOI: https://doi.org/10.1007/s11033-011-0770-x

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