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Anti-apoptotic and antioxidant effect of leptin on CCl4-induced acute liver injury in rats

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Abstract

The aim of this study is to investigate the effect of leptin in rats on carbon tetrachloride (CCl4) induced acute liver damage using immunohistochemical methods for apoptosis and biochemical parameters. In this experimental study, 18 Spraque-Dawley rats were divided into three groups viz; control, CCl4 and CCl4+leptin treatment. 0.8 ml/kg olive oil was administered intraperitoneally (i.p.) to the control group and 0.8 ml/kg CCl4 (1:1 dissolved in olive oil) was administered i.p. to the CCl4 and CCl4+leptin treatment groups, respectively. After 6 h of administrating CCl4, CCl4+leptin treatment group was given i.p. leptin (10 μg/kg). Twenty-four hours after administrating CCl4 all of the groups were euthanized. Biochemical assessments were performed using serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), plasma tumor necrosis factor alpha (TNF-α) levels and tissue malondialdehyde (MDA), and TNF-α levels. Histological assessments were then performed using Hematoxylin&Eosin (H&E) staining in light microscope and apoptosis assessment using Terminal Transferase dUTP Nick End Labeling (TUNEL)-staining. Serum AST, ALT, ALP and plasma TNF-α levels, tissue MDA and TNF-α levels had all increased in CCl4 group, but were found to be significantly decreased in CCl4+leptin treatment group. Moreover, TUNEL-positive cell counts in liver had significantly increased in CCl4 group, but decreased in CCl4+leptin treatment group (P < 0.05). The results of our study the biochemical, histological and TUNEL-staining showed that leptin has treatment effects on liver CCl4 induced injury. It plays a role as a potent free radical scavenger, a powerful antioxidant and it also has anti-apoptotic effects.

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Authors and Affiliations

  1. Department of General Surgery, Eskişehir Osmangazi University, Medical Faculty, Eskisehir, Turkey

    Kerim Şerbetçi & Serdar Erkasap

  2. Vocational School of Health Services, Eskişehir Osmangazi University, Meselik-Eskisehir, 26480, Turkey

    Onur Uysal

  3. Department of Physiology, Eskişehir Osmangazi University, Medical Faculty, Eskisehir, Turkey

    Nilüfer Erkasap

  4. Department of Biochemistry, Medical Faculty, Afyon Kocatepe University, Afyon, Turkey

    Tülay Köken

  5. Department of Biostatistics, Medical Faculty, Eşkisehir Osmangazi University, Eskisehir, Turkey

    Canan Baydemir

Authors
  1. Kerim Şerbetçi
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  2. Onur Uysal
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  3. Nilüfer Erkasap
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  4. Tülay Köken
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  5. Canan Baydemir
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  6. Serdar Erkasap
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Correspondence to Onur Uysal.

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Şerbetçi, K., Uysal, O., Erkasap, N. et al. Anti-apoptotic and antioxidant effect of leptin on CCl4-induced acute liver injury in rats. Mol Biol Rep 39, 1173–1180 (2012). https://doi.org/10.1007/s11033-011-0847-6

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  • DOI: https://doi.org/10.1007/s11033-011-0847-6

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