Skip to main content

Advertisement

SpringerLink
Log in

Association of two porcine reproductive and respiratory syndrome virus (PRRSV) receptor genes, CD163 and SN with immune traits

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

CD163 and sialoadhesin (SN) were reported as two essential receptors for the porcine reproductive and respiratory syndrome virus. To investigate the relationship between these two genes and porcine immunity, we assigned porcine CD163 and SN respectively to SSC5q21-q24 and SSC17q23 by IMpRH. Expression profiles revealed that CD163 and SN were ubiquitously expressed in ten tissues, and were expressed highly in lymph gland, spleen and liver, which implied the potential functions of CD163 and SN in immunity. Moreover, a single nucleotide polymorphism (SNP) c.3534C>T was found in 3′-UTR of the CD163 gene and association analysis showed that this gene was significantly associated with the IgG content in blood (P < 0.05). A novel missense mutation c.878A>G located in exon4 of the SN gene which caused the amino acid transition from histidine to arginine was detected, and it was significantly associated with the WBC count in the peripheral blood (P < 0.05). These results provided fundamental evidence for CD163 and SN as two functional candidate genes affecting immunity in pigs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Wensvoort G, Terpstra C, Pol JM, ter Laak EA, Bloemraad M, de Kluyver EP, Kragten C, van Buiten L, den Besten A, Wagenaar F et al (1991) Mystery swine disease in The Netherlands: the isolation of Lelystad virus. Vet Q 13:121–130

    Article  PubMed  CAS  Google Scholar 

  2. Cavanagh D (1997) Nidovirales: a new order comprising Coronaviridae and Arteriviridae. Arch Virol 142:629–633

    PubMed  CAS  Google Scholar 

  3. Haynes JS, Halbur PG, Sirinarumitr T, Paul PS, Meng XJ, Huffman EL (1997) Temporal and morphologic characterization of the distribution of porcine reproductive and respiratory syndrome virus (PRRSV) by in situ hybridization in pigs infected with isolates of PRRSV that differ in virulence. Vet Pathol 34:39–43

    Article  PubMed  CAS  Google Scholar 

  4. Nauwynck HJ, Duan X, Favoreel HW, Van Oostveldt P, Pensaert MB (1999) Entry of porcine reproductive and respiratory syndrome virus into porcine alveolar macrophages via receptor-mediated endocytosis. J Gen Virol 80(Pt 2):297–305

    PubMed  CAS  Google Scholar 

  5. Calvert JG, Slade DE, Shields SL, Jolie R, Mannan RM, Ankenbauer RG, Welch SK (2007) CD163 expression confers susceptibility to porcine reproductive and respiratory syndrome viruses. J Virol 81:7371–7379

    Article  PubMed  CAS  Google Scholar 

  6. Delputte PL, Vanderheijden N, Nauwynck HJ, Pensaert MB (2002) Involvement of the matrix protein in attachment of porcine reproductive and respiratory syndrome virus to a heparinlike receptor on porcine alveolar macrophages. J Virol 76:4312–4320

    Article  PubMed  CAS  Google Scholar 

  7. Vanderheijden N, Delputte PL, Favoreel HW, Vandekerckhove J, Van Damme J, van Woensel PA, Nauwynck HJ (2003) Involvement of sialoadhesin in entry of porcine reproductive and respiratory syndrome virus into porcine alveolar macrophages. J Virol 77:8207–8215

    Article  PubMed  CAS  Google Scholar 

  8. Delputte PL, Costers S, Nauwynck HJ (2005) Analysis of porcine reproductive and respiratory syndrome virus attachment and internalization: distinctive roles for heparan sulphate and sialoadhesin. J Gen Virol 86:1441–1445

    Article  PubMed  CAS  Google Scholar 

  9. Delputte PL, Nauwynck HJ (2004) Porcine arterivirus infection of alveolar macrophages is mediated by sialic acid on the virus. J Virol 78:8094–8101

    Article  PubMed  CAS  Google Scholar 

  10. Kristiansen M, Graversen JH, Jacobsen C, Sonne O, Hoffman HJ, Law SK, Moestrup SK (2001) Identification of the haemoglobin scavenger receptor. Nature 409:198–201

    Article  PubMed  CAS  Google Scholar 

  11. Van Gorp H, Van Breedam W, Delputte PL, Nauwynck HJ (2009) The porcine reproductive and respiratory syndrome virus requires trafficking through CD163-positive early endosomes, but not late endosomes, for productive infection. Arch Virol 154(12):1939–1943

    Article  PubMed  Google Scholar 

  12. Patton JB, Rowland RR, Yoo D, Chang KO (2009) Modulation of CD163 receptor expression and replication of porcine reproductive and respiratory syndrome virus in porcine macrophages. Virus Res 140(1–2):161–171

    Article  PubMed  CAS  Google Scholar 

  13. Hogger P, Dreier J, Droste A, Buck F, Sorg C (1998) Identification of the integral membrane protein RM3/1 on human monocytes as a glucocorticoid-inducible member of the scavenger receptor cysteine-rich family (CD163). J Immunol 161:1883–1890

    PubMed  CAS  Google Scholar 

  14. Fabriek BO, Dijkstra CD, van den Berg TK (2005) The macrophage scavenger receptor CD163. Immunobiology 210:153–160

    Article  PubMed  CAS  Google Scholar 

  15. Vos JA, Abbondanzo SL, Barekman CL, Andriko JW, Miettinen M, Aguilera NS (2005) Histiocytic sarcoma: a study of five cases including the histiocyte marker CD163. Mod Pathol 18:693–704

    Article  PubMed  Google Scholar 

  16. Harms PW, Bandarchi B, Ma L (2010) CD163 expression in leukemia cutis. J Cutan Pathol 37(9):953–957

    Article  PubMed  Google Scholar 

  17. Zou LY, Peng CQ, Li CZ, Zhao CL, Zhu JM, Liu JL, Zhang CX (2009) Association between hemoglobin scavenger receptor CD163 expression and coronary atherosclerotic severity in patients with coronary heart disease. Zhonghua Xin Xue Guan Bing Za Zhi 37:605–609

    PubMed  CAS  Google Scholar 

  18. Sanchez-Torres C, Gomez-Puertas P, Gomez-del-Moral M, Alonso F, Escribano JM, Ezquerra A, Dominguez J (2003) Expression of porcine CD163 on monocytes/macrophages correlates with permissiveness to African swine fever infection. Arch Virol 148:2307–2323

    Article  PubMed  CAS  Google Scholar 

  19. Crocker PR, Mucklow S, Bouckson V, McWilliam A, Willis AC, Gordon S, Milon G, Kelm S, Bradfield P (1994) Sialoadhesin, a macrophage sialic acid binding receptor for haemopoietic cells with 17 immunoglobulin-like domains. EMBO J 13:4490–4503

    PubMed  CAS  Google Scholar 

  20. Rempel H, Calosing C, Sun B, Pulliam L (2008) Sialoadhesin expressed on IFN-induced monocytes binds HIV-1 and enhances infectivity. PLoS One 3:e1967

    Article  PubMed  Google Scholar 

  21. Crocker PR, Redelinghuys P (2008) Siglecs as positive and negative regulators of the immune system. Biochem Soc Trans 36:1467–1471

    Article  PubMed  CAS  Google Scholar 

  22. Pan PW, Li K, Tuggle CK, Yu M, Liu B, Zhao SH (2003) Sequencing, tissue distribution and physical mapping of the porcine homologue of cardiomyopathy associated 3 (CMYA3). Anim Genet 34:473–474

    Article  PubMed  CAS  Google Scholar 

  23. Yerle M, Pinton P, Robic A, Alfonso A, Palvadeau Y, Delcros C, Hawken R, Alexander L, Beattie C, Schook L, Milan D, Gellin J (1998) Construction of a whole-genome radiation hybrid panel for high-resolution gene mapping in pigs. Cytogenet Cell Genet 82:182–188

    Article  PubMed  CAS  Google Scholar 

  24. Miner JL (2004) The adipocyte as an endocrine cell. J Anim Sci 82(3):935–941

    PubMed  CAS  Google Scholar 

  25. Lord GM, Matarese G, Howard JK, Baker RJ, Bloom SR, Lechler RI (1998) Leptin modulates the T-cell immune response and reverses starvation-induced immunosuppression. Nature 394(6696):897–901

    Article  PubMed  CAS  Google Scholar 

  26. Huang H, Zhao W, Tang Z, Yang S, Wu Z, Zhao S, Cui W, Mu Y, Chu M, Li K (2009) Characterization of porcine MMP-2 and its association with immune traits. Gene 435:63–71

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

The authors would like to thank Dr. Martine Yerle for providing the SCHP and RH panel (INRA, Castanet-Tolosan, France). The work was supported by Major Program of Natural Science Foundation of Hubei, Doctoral Fund of Ministry of Education of China (20100146110021), Scientific Research Foundation for Returned Scholars, Ministry of Education of China and the creative team project of Education Ministry of China (IRT0831).

Author information

Authors and Affiliations

  1. Laboratory of Molecular Biology and Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China

    Fengli Wang, Haifang Qiu, Qingde Zhang, Zhongzhen Peng & Bang Liu

Authors
  1. Fengli Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Haifang Qiu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Qingde Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zhongzhen Peng
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Bang Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Bang Liu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, F., Qiu, H., Zhang, Q. et al. Association of two porcine reproductive and respiratory syndrome virus (PRRSV) receptor genes, CD163 and SN with immune traits. Mol Biol Rep 39, 3971–3976 (2012). https://doi.org/10.1007/s11033-011-1177-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-011-1177-4

Keywords

Search

Navigation