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Renin-angiotensin system polymorphisms in relation to hypertension status and obesity in a Tunisian population

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Abstract

Essential hypertension (HTA) is the clinical expression of a disordered interaction between the genetic, physiological, and biochemical systems that under usual conditions maintain cardiovascular homeostasis. We studied the effects of the angiotensinogen M235T, angiotensin converting enzyme insertion/deletion (ACE I/D), and angiotensin II receptor 1 (AT1R) A1166C gene polymorphisms on the risk of HTA and to evaluate the relationship between these polymorphisms and obesity. We performed AGT, ACE and AGTR genotyping in 142 hypertensive patients and 191 control subjects using PCR-RFLP methods and PCR, respectively. The three polymorphisms were significantly associated with HTA. Individuals carrying the mutated TT of AGT, DD of ACE and CC of AT1R genotypes had an 1.67 (P = 0.032), 3.09 (P < 0.001) and 3.45 (P < 0.001)-fold increased risk of HTA. After adjustment for sex, smoking, diabetes, dyslipidemia, BMI, triglycerides and DD, TT and CC genotypes, BMI was independent risk factor of HTA (OR = 3.14; P < 0.001). An association of BMI with ACE gene polymorphism (P = 0.035), whereas no association with AGT and AT1R gene polymorphisms was obtained. The proportion of hypertensives is as high as 21.8 and 13.4% in the overweight and the obese DD group. The present study implies that the genotyping for the variants of RAS gene could in the future become an important part of the clinical process of risk identification for HTA.

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Acknowledgments

This work was supported by grants from “Ministère de l’Enseignement Supérieur, de la Recherche Scientifique et de la Technologie “Nutrition Humaine et Désordres Métaboliques” and “Direction Générale de la Recherche Scientifique et Technologique” DGRST-USCR Spectrométrie de masse.

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Authors and Affiliations

  1. Laboratory of Nutrition and Vascular Health, Faculty of Medicine, Monastir, Tunisia

    Sounira Mehri, Sinda Mahjoub, Sonia Hammami, Ameur Frih & Mohamed Hammami

  2. Department of Internal Medicine, University Hospital “F. Bourguiba”, Monastir, Tunisia

    Sonia Hammami

  3. Department of Cardiology, LaRabta, Tunis, Tunisia

    Amira Zaroui & Rachid Mechmeche

  4. Department of Cardiology, University Hospital “F. Bourguiba”, Monastir, Tunisia

    Fathi Betbout

Authors
  1. Sounira Mehri
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  2. Sinda Mahjoub
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  3. Sonia Hammami
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  4. Amira Zaroui
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  7. Rachid Mechmeche
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  8. Mohamed Hammami
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Correspondence to Sounira Mehri.

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Mehri, S., Mahjoub, S., Hammami, S. et al. Renin-angiotensin system polymorphisms in relation to hypertension status and obesity in a Tunisian population. Mol Biol Rep 39, 4059–4065 (2012). https://doi.org/10.1007/s11033-011-1187-2

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  • DOI: https://doi.org/10.1007/s11033-011-1187-2

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