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Preventive role of gallic acid on alcohol dependent and cysteine protease-mediated pancreas injury

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Abstract

In order to investigate an association between alcohol consumption and lysosomal cysteine protease induced pancreatic injury and preventive effect of gallic acid as dose-dependent, we determined myeloperoxidase and malondialdehyde levels, serum amylase activities and cathepsin B and L activities in the cytosolic and lysosomal fractions of pancreatic tissue in the ethanol (8 g/kg) and ethanol plus gallic acid (at different doses 50, 100 and 200 mg/kg) given rats. Absolute ethanol (8 g/kg) was given by oral gavage. Gallic acid was dissolved in the saline (2 ml/kg) and administered before 30 min the oral administration of ethanol. Pancreatic myeloperoxidase and also malondialdehyde levels and serum amylase activities were measured. Besides, histological investigations were made. Cathepsin B activities in the cytosolic fraction were decreased by gallic acid (200 mg/kg) and increased in ethanol given rats. Cytosolic/lysosomal ratio of cathepsin B and L were found to be low in the all doses of gallic acid as compared to ethanol group. Serum amylase, pancreatic myeloperoxidase activities and malondialdehyde levels in the ethanol group were higher than in the control group. These were not statistically significant for myeloperoxidase and malondialdehyde. Also, our histopathologic results indicated that ethanol administration increased pancreatic tissue injury. Gallic acid especially at 200 mg/kg improved ethanol-mediated pancreatic tissue damage.In conclusion, gallic acid treatments were decreased release of lysosomal cathepsin B and L enzymes into cytoplasmic fraction and prevented alcohol mediated pancreatic tissue injury. Preventive effect of gallic acid might be dose-dependent.

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Abbreviations

MPO:

Myeloperoxidase

MDA:

Malondialdehyde

FAEE:

Fatty acid ethyl esters

C:

Cytosolic

L:

Lysosomal

C/L:

Cytosolic/lysosomal

FEL:

Fractions enriched with lysosomes

SPSS:

Statistical package for social sciences

CYP2E1:

Cytochrome P4502E1

PMN:

Polymorphonuclear

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The authors have declared no conflict of interest

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Medicine, Osmangazi University, Eskisehir, 26480, Turkey

    Güngör Kanbak, Ayşegül Oğlakçı & Kazim Kartkaya

  2. Department of Biology, Faculty of Art and Science, Osmangazi University, Eskisehir, Turkey

    Mediha Canbek, Hakan Şentürk, Burak Göl & Ayşe Özmen

  3. Department of Zoology, Faculty of Art and Science, Artvin Coruh University, Artvin, Turkey

    Gökhan Bayramoğlu

  4. Department of Biostatistics, Faculty of Medicine, Osmangazi University, Eskisehir, Turkey

    Cengiz Bal

Authors
  1. Güngör Kanbak
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  2. Mediha Canbek
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  3. Ayşegül Oğlakçı
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  4. Kazim Kartkaya
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  7. Cengiz Bal
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  9. Ayşe Özmen
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Correspondence to Kazim Kartkaya.

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Kanbak, G., Canbek, M., Oğlakçı, A. et al. Preventive role of gallic acid on alcohol dependent and cysteine protease-mediated pancreas injury. Mol Biol Rep 39, 10249–10255 (2012). https://doi.org/10.1007/s11033-012-1901-8

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  • DOI: https://doi.org/10.1007/s11033-012-1901-8

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