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Monocytes expression of IL-12 related and IL-10 genes in association with development of colorectal cancer

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Abstract

The main regulator of anti-tumor immune response is the activity of monocytes, suggesting that the produced cytokines may have a prognostic role. This study investigates gene expression of interleukin (IL)-12-related cytokine and IL-10 in stimulated monocytes from colorectal cancer (CRC) patients. Relative quantification of IL-12A, IL-12B, IL-23A and IL-10 mRNA transcripts was performed on the third hours after stimulation by real-time qPCR. We also explored an inhibitor of JNK signaling pathway activation for the observed cytokine gene expression. A strong downregulation of IL-12B mRNA expression in CRC monocytes compared to healthy donors was observed. The rate of transcription of IL-12B in stimulated monocytes was associated with the stage of CRC. The expression of IL-12A gene in stimulated monocytes from patients with advanced was lower than early cancer. Moreover, we observed stage dependent JNK inhibition mediated reduction in IL-12A expression. The hyporesponsiveness was strongly expressed in monocytes from advanced then early stages of CRC. Expression of IL-10 mRNA was almost equally in CRC monocytes from early stages and healthy donors. We demonstrated that altered gene expression profiles of IL-12A, IL-12B, IL-23A at mRNA level in CRC monocytes was associated with tumor development and can be attributed to anticancer immune response.

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Acknowledgments

This work was supported by Grant No: 7/2010 from the Fund for Scientific and Mobile project from Faculty of Medicine at the Trakia University, Stara Zagora, Bulgaria.

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Authors and Affiliations

  1. Department of Neurosurgery, Surgery and Urology, University Hospital, Medical Faculty, Trakia University, Stara Zagora, Bulgaria

    Noyko S. Stanilov, Jovcho P. Jovchev & Geo M. Cirovski

  2. BMI Healthcare, The Harbour Hospital, St Mary’s Road, Poole, Dorset, UK

    Noyko S. Stanilov

  3. Department of Molecular Biology, Immunology & Medical Genetics, Faculty of Medicine, Trakia University, Aremiska 11 Str., Stara Zagora, Bulgaria

    Lyuba D. Miteva, Zlatka G. Dobreva & Spaska A. Stanilova

Authors
  1. Noyko S. Stanilov
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  2. Lyuba D. Miteva
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  3. Zlatka G. Dobreva
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  4. Jovcho P. Jovchev
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  5. Geo M. Cirovski
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  6. Spaska A. Stanilova
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Correspondence to Spaska A. Stanilova.

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Stanilov, N.S., Miteva, L.D., Dobreva, Z.G. et al. Monocytes expression of IL-12 related and IL-10 genes in association with development of colorectal cancer. Mol Biol Rep 39, 10895–10902 (2012). https://doi.org/10.1007/s11033-012-1987-z

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  • DOI: https://doi.org/10.1007/s11033-012-1987-z

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