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DNA methylation status of the methylenetetrahydrofolate reductase gene promoter in peripheral blood of end-stage renal disease patients

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Abstract

End-stage renal disease (ESRD) is one of the main causes of morbidity and mortality worldwide. DNA methylation is a major epigenetic modification of the genome that has the potential to silence gene expression. Methylenetetrahydrofolate reductase (MTHFR) gene inactivation was recognized as a predisposing factor of hyperhomocysteinemia in renal patients. The current study aimed to determine the methylation status within the MTHFR promoter region in DNA isolated from peripheral blood of ESRD patients and controls and the correlation of this methylation with the clinical and biochemical characteristics in ESRD patients. Ninety-six ESRD patients and 96 healthy ethnically, age and gender matched controls were included within the study. MTHFR promoter methylation was assessed using methylation specific polymerase chain reaction. The frequency of MTHFR methylation was significantly higher in ESRD patients than in controls (P = 0.003), additionally, MTHFR methylation was associated to a decrease in estimated glomerular filtration rate, serum high-density lipoprotein cholesterol level and an increase in both serum total cholesterol and low-density lipoprotein cholesterol levels. Data generated from this study suggest the possible involvement of MTHFR promoter methylation in the pathogenesis of ESRD and support a new dimension of MTHFR inactivation.

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Authors and Affiliations

  1. Department of Medical Biochemistry, Faculty of Medicine, Suez Canal University, Ismailia, Egypt

    Maivel Ghattas

  2. Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Industries, Sinai University, North Sinai, Egypt

    Fatma El-shaarawy

  3. Department of Biochemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, 41622, Egypt

    Noha Mesbah & Dina Abo-Elmatty

Authors
  1. Maivel Ghattas
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  2. Fatma El-shaarawy
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  3. Noha Mesbah
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  4. Dina Abo-Elmatty
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Correspondence to Dina Abo-Elmatty.

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Ghattas, M., El-shaarawy, F., Mesbah, N. et al. DNA methylation status of the methylenetetrahydrofolate reductase gene promoter in peripheral blood of end-stage renal disease patients. Mol Biol Rep 41, 683–688 (2014). https://doi.org/10.1007/s11033-013-2906-7

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  • DOI: https://doi.org/10.1007/s11033-013-2906-7

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