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Association of the miR-146aC>G, miR-149T>C, and miR-196a2T>C polymorphisms with gastric cancer risk and survival in the Greek population

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Abstract

MicroRNAs (miRNAs) play an important role in regulating gene expression at the post-transcriptional level and are involved in numerous physiological processes. Accumulating evidence suggests that single-nucleotide polymorphisms (SNPs) in human miRNA genes may affect miRNA biogenesis pathway and influence the susceptibility to several diseases such as cancer. The aim of the study was to investigated whether three common miRNA polymorphisms [miR-146a C>G (rs2910164), miR-149 T>C (rs2292832), and miR-196a2 T>C (rs11614913)] are associated with the susceptibility and prognosis of gastric cancer (GC) in the Greek population. The three mRNA SNPs were identified in a case–control study (163 patients; 480 controls) by polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) analysis. We found that the risk for GC was significantly higher for the carriers of miR-149 rs2292832CC (p = 0.009) and miR-196a2 rs11614913CC (p < 0.0001) genotypes, as well as for the carriers of the rs2910164/rs2292832/rs11614913 CCC and GTC haplotype (p < 0.0001 and p = 0.03, respectively). The rs2910164/rs2292832/rs11614913 CTT and CCT haplotypes seems to have a protective role against GC (p = 0.002 and p = 0.001, respectively). Our data demonstrate that specific miRNA SNPs are associated with GC susceptibility in the Greek population.

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Authors and Affiliations

  1. First Surgical Department, “Tzaneion” Hospital, Piraeus, Greece

    Panagiotis Dikeakos & Spyros Rizos

  2. First Propaedeutic Surgical Department, School of Medicine, Hippocratio General Hospital, Athens, Greece

    George Theodoropoulos & George Zografos

  3. Surgical Department, Asclepieion Voulas, Athens, Greece

    Nikolaos Tzanakis

  4. Department of Basic Medical Sciences, Laboratory of Biology, School of Medicine, University of Athens, Michalakopoulou 176, 11527, Athens, Greece

    Maria Gazouli

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  1. Panagiotis Dikeakos
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  2. George Theodoropoulos
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  3. Spyros Rizos
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  4. Nikolaos Tzanakis
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  5. George Zografos
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  6. Maria Gazouli
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Correspondence to Maria Gazouli.

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Dikeakos, P., Theodoropoulos, G., Rizos, S. et al. Association of the miR-146aC>G, miR-149T>C, and miR-196a2T>C polymorphisms with gastric cancer risk and survival in the Greek population. Mol Biol Rep 41, 1075–1080 (2014). https://doi.org/10.1007/s11033-013-2953-0

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  • DOI: https://doi.org/10.1007/s11033-013-2953-0

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