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Profiles of serum microRNAs; miR-125b-5p and miR223-3p serve as novel biomarkers for HBV-positive hepatocellular carcinoma

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Abstract

Recently, circulating miRNAs have been reported as promising biomarkers for various pathologic conditions including cancer. Certain microRNAs (miRNAs) have been shown early diagnostic potential for many types of cancer. The objective of this study was to investigate the potential of certain serum/plasma miRNAs as novel non-invasive biomarkers for early diagnosis of hepatitis B virus (HBV) related hepatocellular carcinoma (HCC). For this reason, the expression levels of 24 miRNA (let-7c, miR-92a-3p, 423-5p, 150-5p, 223-3p, 125b-5p, 342-3p, miR-206, 122-5p, 375, 223-5p, 10a-5p, 23b-5p, 99a-5p, 23a-5p, 10a-3p, 122-3p, 125b-1-3p, 23b-3p, 125b-2-3p, 23a-3p, 92a-1-5p, 92a-2-5p, 99a-3p) were analyzed in plasma of patients with chronic hepatitis B, HBV-positive cirrhosis and HBV-positive HCC and compared with control group samples. Totally 94 plasma samples; 28 control and 66 patient plasma (24 CHB, 22 HBV-positive cirrhosis, 20 HBV-positive HCC) and were included in this study. The expression levels of 24 miRNAs were detected for all control and patient group plasma samples by qRT-PCR using BioMark™ 96.96 Dynamic Array (Fluidigm Corporation) system. The expression levels of miR-125b-5p were detected 2.85 fold, 2.46 fold and 1.89 fold (p = 0.01513, p = 0.0009440, p = 0.0001446) up regulated in CHB, HBV-positive cirrhosis and HBV-positive HCC, respectively when compared versus control group individually by Mann–Whitney U test. The expression levels of miR-223-3p were detected 5.55 fold, 13.88 fold and 12.65 fold (p = 0.01513, p = 0.0009440, p = 0.0001446) down regulated in same comparisons. When all groups were compared versus control group by one-way ANOVA test, the expression levels of miR-223-3p were also found statistically significant (p < 0.05). Although not statistically significant, miR-125b-5p tended to be upregulated. (p = 0.07192). These results significantly imply that miR-125b-5p and miR223-3p could be used as novel non-invasive biomarkers of HBV-positive HCC in very early, even at CHB stage of liver disease.

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Acknowledgments

This study was supported by Grants from the Mersin University Scientific Research Funds, Mersin, Turkey [Project no. BAP-SBE TM (BGG) 2011-5 YL].

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Authors and Affiliations

  1. Department of Medical Microbiology, Faculty of Medicine, Mersin University, Mersin, Turkey

    Burcu Gurer Giray, Gurol Emekdas, Seda Tezcan & Mahmut Ulger

  2. Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Mersin University, Yenisehir, 33169, Mersin, Turkey

    Mehmet Sami Serin

  3. Department of Gastroenterology, Faculty of Medicine, Mersin University, Mersin, Turkey

    Orhan Sezgin & Engin Altintas

  4. Department of Hematology, Faculty of Medicine, Mersin University, Mersin, Turkey

    Eyup Naci Tiftik

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  1. Burcu Gurer Giray
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  2. Gurol Emekdas
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  4. Mahmut Ulger
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  5. Mehmet Sami Serin
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Correspondence to Mehmet Sami Serin.

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Giray, B.G., Emekdas, G., Tezcan, S. et al. Profiles of serum microRNAs; miR-125b-5p and miR223-3p serve as novel biomarkers for HBV-positive hepatocellular carcinoma. Mol Biol Rep 41, 4513–4519 (2014). https://doi.org/10.1007/s11033-014-3322-3

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  • DOI: https://doi.org/10.1007/s11033-014-3322-3

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