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PCBP1 is an important mediator of TGF-β-induced epithelial to mesenchymal transition in gall bladder cancer cell line GBC-SD

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Abstract

Gall bladder carcinoma (GBC) is the seventh most common cancer across the globe and the most common malignancy of the biliary tract. Most GBC related deaths occur due to secondary progression and metastasis to distant organs. Epithelial–mesenchymal transition (EMT) is an important pre-requisite for tumor metastasis, however its mechanism in GBC has not yet been defined. Using the GBC-SD cell line, we have uncovered an important mediator, poly r(C) binding protein-1 (PCBP1), of transforming growth factor-beta (TGF-β)-induced EMT in GBC. Our results show that TGF-β treatment resulted in PCBP1 phosphorylation in accordance with similar observation in other model systems. We further showed through gain- and loss-of-function assays that PCBP1 expression levels regulate the capacity of GBC-SD cells to migrate and invade in vitro. Finally, our results showed that PCBP1 expression levels also regulate generation of CD44+CD24− progenitor cell population in GBC-SD cells after TGF-β treatment. Cumulatively, our results indicate, pending further validation, that PCBP1 might be a prognostic marker for GBC metastasis.

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Abbreviations

GBC:

Gall bladder carcinoma

EMT:

Epithelial–mesenchymal transition

PCBP1:

Poly r(C) binding protein-1

TGF-β:

Transforming growth factor-beta

FBS:

Fetal bovine serum

EDTA:

Ethylene diamine tetraacetic acid

PBS:

Phosphate buffered saline

BSA:

Bovine serum albumin

FITC:

Fluorescein isothiocyanate

PE:

Phycoerythrin

PI:

Propidium-iodide

SEM:

Standard error of mean

SICs:

Spreading initiation centers

FcGBP:

Fc fragment of the IgG binding protein

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Authors and Affiliations

  1. Department of Hepatobiliary Surgery, The Fourth Military Medical University Xijing Hospital, No. 15 Changlexi Road, Xi’an City, 710032, Shaanxi Province, China

    Hang-Yu Zhang & Ke-Feng Dou

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  1. Hang-Yu Zhang
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  2. Ke-Feng Dou
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Correspondence to Ke-Feng Dou.

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Zhang, HY., Dou, KF. PCBP1 is an important mediator of TGF-β-induced epithelial to mesenchymal transition in gall bladder cancer cell line GBC-SD. Mol Biol Rep 41, 5519–5524 (2014). https://doi.org/10.1007/s11033-014-3428-7

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  • DOI: https://doi.org/10.1007/s11033-014-3428-7

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