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Primary Culture of Dissociated Cells of the Rat Retina under Conditions of Long-Lasting Culturing: Properties of Ganglion Cells

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Neurophysiology Aims and scope

We developed a technique for long-lasting culturing of dissociated cells of the rat retina. Electrophysiological characteristics of cultured retinal ganglion cells were examined using a patch-clamp technique in the whole-cell configuration (current-clamp mode). Morphological and electrophysiological characteristics of cultured retinal ganglion cells corresponded to those obtained under conditions of an acute experiment on the cells of similar-age animals; this is indicative of the possibility of using cultured retinal cells in electrophysiological and pharmacological studies.

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Authors and Affiliations

  1. Bogomolets Institute of Physiology of the National Academy of Sciences of Ukraine, Kyiv, Ukraine

    G. V. Dumanskaya, H. E. Purnyn, O. V. Rykhal’skii & N. S. Veselovskii

Authors
  1. G. V. Dumanskaya
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  2. H. E. Purnyn
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  3. O. V. Rykhal’skii
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  4. N. S. Veselovskii
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Corresponding author

Correspondence to G. V. Dumanskaya.

Additional information

Neirofiziologiya/Neurophysiology, Vol. 43, No. 4, pp. 369–371, July-August, 2011.

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Dumanskaya, G.V., Purnyn, H.E., Rykhal’skii, O.V. et al. Primary Culture of Dissociated Cells of the Rat Retina under Conditions of Long-Lasting Culturing: Properties of Ganglion Cells. Neurophysiology 43, 321–323 (2011). https://doi.org/10.1007/s11062-011-9223-3

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  • DOI: https://doi.org/10.1007/s11062-011-9223-3

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