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Microarray Analysis of Gene Expression in Rat Cortical Neurons Exposed to Hyperbaric Air and Oxygen

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Abstract

To gain a global view of the genomic response of neurons to normobaric and hyperbaric hyperoxic stress, we performed a microarray analysis of gene expression after exposure to varying levels of partial oxygen pressures. Rat neurons were exposed to normobaric hyperoxia, hyperbaric (2, 4, and 6 atmosphere absolute) air or hyperbaric O2. We identified 183 genes significantly altered (increased or decreased ≥1.5-fold) in response to pressure and/or oxidative stress. Among them, 17 genes changed in response to all exposure conditions. More genes were altered in response to hyperbaric air than hyperbaric O2. The altered genes included factors associated with stress responses, transport/neurotransmission, signal transduction, and transcription factors. The results may serve as guidance for selection of biomarkers of hyperoxia and hyperbaric O2 response and provide a starting point for further studies to investigate the global molecular mechanisms underlying hyperbaric oxidative stress.

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Acknowledgments

The authors are grateful to Mr. John Boal for technical support and Ms. Diana Temple for editorial assistance. This work was supported by Office of Naval Research Work Unit #0601153N.4118.A0303. The opinions expressed in this presentation are those of the authors and do not reflect the official policy of the Department of Navy, Department of Defense, of the U.S. Government.

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Authors and Affiliations

  1. Undersea Medicine Department, Naval Medical Research Center, 503 Robert Grant Ave, Bldg 503/Rm 1A10, Silver Spring, MD, 20910, USA

    Ye Chen, N. Suzan Nadi, Mikulas Chavko, Charles R. Auker & Richard M. McCarron

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  1. Ye Chen
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  2. N. Suzan Nadi
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  3. Mikulas Chavko
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Correspondence to Richard M. McCarron.

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Ye Chen and N. Suzan Nadi have contributed equally to this work.

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Chen, Y., Nadi, N.S., Chavko, M. et al. Microarray Analysis of Gene Expression in Rat Cortical Neurons Exposed to Hyperbaric Air and Oxygen. Neurochem Res 34, 1047–1056 (2009). https://doi.org/10.1007/s11064-008-9873-8

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