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Dimethylarginine Levels in Cerebrospinal Fluid of Hyperacute Ischemic Stroke Patients are Associated with Stroke Severity

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Abstract

We hypothesise that asymmetric and symmetric dimethylarginine (ADMA, SDMA) are released in cerebrospinal fluid (CSF) due to ischemia-induced proteolysis and that CSF dimethylarginines are related to stroke severity. ADMA and SDMA were measured in CSF of 88 patients with ischemic stroke or TIA within 24 h after stroke onset (mean 8.6 h) and in 24 controls. Stroke severity was assessed by the National Institutes of Health Stroke Scale (NIHSS) score at admission. Outcome was evaluated by institutionalization due to stroke and the modified Rankin scale. Dimethylarginine levels were higher in patients with stroke than in TIA patients, who had higher levels than controls and correlated with the NIHSS. Logistic regression analysis confirmed that dimethylarginines were independently associated with stroke severity. The SDMA/ADMA ratio did not differ significantly between controls and stroke patients. CSF dimethylarginine levels are increased in hyperacute ischemic stroke and are associated with stroke severity.

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Abbreviations

ADMA:

Asymmetric dimethylarginine

CI:

Confidence interval

CSF:

Cerebrospinal fluid

CT:

Computed tomography

Exp(β):

Exponential β

MDRD:

Modification of diet in renal diseases formula

mRS:

Modified Rankin scale

MRI:

Magnetic resonance imaging

NIHSS:

National Institutes of Health Stroke Scale

SDMA:

Symmetric dimethylarginine

TDMA:

Total dimethylarginine

TIA:

Transient ischemic attack

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Acknowledgments

R. B. is a research assistant of the Fund for Scientific research Flanders (FWO-Vlaanderen). This research was also supported by the Institute Born-Bunge; the agreement between the Institute Born-Bunge and the University of Antwerp; the Interuniversity Attraction Poles (IAP) program P6/43 of the Belgian Federal Science Policy Office, Belgium; and the Medical Research Foundation Antwerp.

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Authors and Affiliations

  1. Department of Neurology, University Hospital Brussels, Vrije Universiteit Brussel, Brussels, Belgium

    Raf Brouns

  2. Department of Neurology and Memory Clinic, ZNA Middelheim Hospital, Antwerp, Belgium

    Raf Brouns, Rishi Sheorajpanday & Peter P. De Deyn

  3. Laboratory for Neurochemistry and Behaviour, Institute Born-Bunge, Department of Biomedical Sciences, University of Antwerp, Universiteitsplein 1, 2610, Antwerp, Belgium

    Raf Brouns, Bart Marescau, Ilse Possemiers, Rishi Sheorajpanday & Peter P. De Deyn

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  1. Raf Brouns
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  2. Bart Marescau
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Correspondence to Peter P. De Deyn.

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R. Brouns is a research assistant of the Fund for Scientific research Flanders (FWO-Vlaanderen).

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Brouns, R., Marescau, B., Possemiers, I. et al. Dimethylarginine Levels in Cerebrospinal Fluid of Hyperacute Ischemic Stroke Patients are Associated with Stroke Severity. Neurochem Res 34, 1642–1649 (2009). https://doi.org/10.1007/s11064-009-9954-3

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  • DOI: https://doi.org/10.1007/s11064-009-9954-3

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