Abstract
In Parkinson’s disease, dopaminergic neuron damage/death causes the release of soluble substances that are selectively toxic to neighboring/additional dopaminergic neurons through the activation of microglia. Hsp60 can be released from injured cells of central nervous system to activate microglia. However, its expression and role in Parkinson’s disease has not been well understood. Here, we performed a 6-OHDA treated Parkinson’s disease model in adult rats. Western blot analysis showed a time-course expression of Hsp60, which decreased gradually and then rose back. Immunofluorescence staining showed that Hsp60 was decreased in dopaminergic neuron, and most Hsp60 located on the surface of activated microglia. Furthermore, in cellular Parkinson’s disease model, Hsp60 was obviously detected in the culture supernatants after 6-OHDA treatment, and a concomitant decrease in cell extracts. Taken together, our results suggested that Hsp60 could be released extracellularly to activate microglia in Parkinson’s disease model.
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Abbreviations
- Hsp60:
-
Heat shock protein 60
- 6-OHDA:
-
6-Hydroxydopamine
- PD:
-
Parkinson’s disease
- CNS:
-
Central nervous system
- SNpc:
-
Substantia nigra pars compacta
- TLR-4:
-
Toll like receptor-4
- NF-κB:
-
Nuclear factor kappa B
- IKK:
-
I-κB kinase
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Acknowledgments
This work was supported by Natural Science Foundation of Jiangsu province (No. BK2008480) and ‘‘Six Talent Peak’’ Foundation of Jiangsu province (2010-WS-030).
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Mei jiang Feng and Ling Zhang contributed equally to this work.
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11064_2013_1127_MOESM1_ESM.tif
TH immunoreactivity in the whole SN at sham control (a), 6-OHDA 1 week (b), and 6-OHDA 4 week (c) by immunohistochemistry. There was a progressive loss of dopaminergic neurons in the SN of rats injected with 6-OHDA. Scale bar 50μm (TIFF 2129 kb)
11064_2013_1127_MOESM2_ESM.tif
Quantitative analysis of CD11b positive cells expressing Hsp60 (%) in control group, 1, 2 and 4 week after 6-OHDA injection. *P < 0.05 as compared with the control group. Error bars represent SEM (TIFF 631 kb)
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Feng, M.j., Zhang, L., Liu, Z. et al. The Expression and Release of Hsp60 in 6-OHDA Induced In Vivo and In Vitro Models of Parkinson’s Disease. Neurochem Res 38, 2180–2189 (2013). https://doi.org/10.1007/s11064-013-1127-8
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DOI: https://doi.org/10.1007/s11064-013-1127-8