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Forsythiaside Protects Against Hydrogen Peroxide-Induced Oxidative Stress and Apoptosis in PC12 Cell

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Abstract

Oxidative stress is a major component of harmful cascades activated in neurodegenerative disorders. We sought to elucidate possible effects of forsythiaside, an active constituent isolated from the Chinese medicinal herb Forsythia suspense, on hydrogen peroxide (H2O2)-induced cell death and to determine the underlying molecular mechanisms in neuron-like PC12 cells. We found that forsythiaside treatment effectively protected PC12 cells against H2O2-induced cell damage and apoptosis. H2O2 exposure induced oxidative stress in PC12 cells, as revealed by increased ROS and lipid peroxidation (MDA), which were inhibited by forsythiaside pretreatment. Increased Bax/Bcl-2 ratio, mitochondrial membrane potential decrease, cytochrome c release, caspase-9/-3 activation, AIF/Endo G translocation were observed in H2O2-treated cells. Interestingly, forsythiaside effectively prevented these events. These results suggested that forsythiaside prevented H2O2-induced mitochondria-dependent apoptosis. Further, increased nuclear levels of Nrf2 and up-regulation of antioxidant enzymes (Mn/SOD and CAT) were detected in forsythiaside-treated cells, indicating the anti-oxidative effects of forsythiaside might be associated with activation of Nrf2 pathway. Moreover, forsythiaside was proved to be effective to prevent LPS-induced cell death and ROS generation. In conclusion, forsythiaside effectively inhibited H2O2-induced oxidative stress and subsequent apoptosis activation.

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The authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. School of Public Health, Anhui Medical University, 81 Meishan Rd., Hefei, 230032, Anhui Province, China

    ChunKang Huang, Hong Su & Dongqing Ye

  2. Fujian University of TCM, 1 Qiuyang Rd., Fuzhou, 350122, Fujian Province, China

    Yonglian Lin

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  1. ChunKang Huang
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  2. Yonglian Lin
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  3. Hong Su
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  4. Dongqing Ye
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Correspondence to Dongqing Ye.

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Huang, C., Lin, Y., Su, H. et al. Forsythiaside Protects Against Hydrogen Peroxide-Induced Oxidative Stress and Apoptosis in PC12 Cell. Neurochem Res 40, 27–35 (2015). https://doi.org/10.1007/s11064-014-1461-5

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  • DOI: https://doi.org/10.1007/s11064-014-1461-5

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