Abstract
Myosin phosphatase (MP) is a major phosphatase responsible for the dephosphorylation of the regulatory light chain of myosin II. MYPT1, a target subunit of smooth and nonmuscle MP, is responsible for activation and regulation of MP. To identity the physiological roles of MP, we have generated MYPT1-deficient mice by gene targeting. The heterozygous mice showed no changes in expression levels of MYPT1 and no distinct phenotype compared to wild-type mice was observed. None of the F2 mice were homozygous for the MYPT1 deletion, indicating that the targeted disruption of the MYPT1 gene resulted in embryonic lethality. The point of embryonic lethality is before 7.5 dpc. These findings indicate that MYPT1 is essential for mouse embryogenesis.
Similar content being viewed by others
References
PTW Cohen (2002) ArticleTitleProtein phosphatase 1 – targeted in many directions J Cell Sci 115 241–256 Occurrence Handle11839776
DJ Hartshorne (1987) Biochemistry of the contractile process in smooth muscle LR Johnson (Eds) Physiology of the Gastrointestinal Tract Raven Press New York 432–482
DJ Hartshorne M Ito F Erdödi (2004) ArticleTitleRole of protein phosphatase type 1 in contractile functions: Myosin phosphatase J Biol Chem 279 37211–37214 Occurrence Handle15136561
M Ito T Nakano F Erdödi DJ Hartshorne (2004) ArticleTitleMyosin phosphatase: structure, regulation and function Mol Cell Biochem 259 197–209 Occurrence Handle15124925
H Machida M Ito R Okamoto K Shiraki N Isaka DJ Hartshorne T Nakano (2001) ArticleTitleMolecular cloning and analysis of the 5′-flanking region of the human MYPT1 gene Biochem Biophys Acta 1517 424–429 Occurrence Handle11342221
T Mizuno K Tsutsui Y Nishida (2002) ArticleTitleDrosophila myosin phosphatase and its role in dorsal closure Development 129 1215–1223 Occurrence Handle11874917
AP Somlyo AV Somlyo (2003) ArticleTitleCa2+ sensitivity of smooth muscle and nonmuscle myosin II: modulated by G proteins, kinases, and myosin phosphatase Physiol Rev 83 1325–1358 Occurrence Handle14506307
C Tan B Stronach N Perrimon (2003) ArticleTitleRoles of myosin phosphatase during Drosophila Development 130 671–681 Occurrence Handle12505998
A Wissmann J Ingles JD McGhee PE Mains (1997) ArticleTitleCaenorhabditis elegans LET-502 is related to Rho-binding kinases and human myotonic dystrophy kinases and interacts genetically with a homolog of the regulatory subunit of smooth muscle myosin phosphatase to affect cell shape Gene Dev 11 409–422 Occurrence Handle9042856
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Okamoto, R., Ito, M., Suzuki, N. et al. The targeted disruption of the MYPT1 gene results in embryonic lethality. Transgenic Res 14, 337–340 (2005). https://doi.org/10.1007/s11248-005-3453-3
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11248-005-3453-3