Skip to main content
SpringerLink
Log in

First report of Porcine teschovirus (PTV), Porcine sapelovirus (PSV) and Enterovirus G (EV-G) in pig herds of Brazil

  • Regular Articles
  • Published:
Tropical Animal Health and Production Aims and scope Submit manuscript

Abstract

Porcine teschovirus (PTV), Porcine sapelovirus (PSV) and Enterovirus G (EV-G) have been associated with enteric, respiratory, reproductive and neurological disorders. Although Brazil is the world’s fourth largest producer and exporter of pork, no information on the occurrence of PTV, PSV and EV-G infections is available for Brazilian pig herds. This study aimed to investigate the occurrence of Porcine enteric picornavirus infections in pig farms located in three distinct geographical regions of Brazil. Forty randomly selected diarrhoeic and normal consistency faeces of suckling (n = 22) and nursery (n = 18) pigs from farms located in 21 distinct cities of the Southern, Southeast, and Midwest regions of Brazil were evaluated by nested-RT-PCR assays. Suckling piglets presented the expected amplicon size for PTV (158 bp) and EV-G (313 bp) in single and mixed infections in 40.9 % (9/22) of the faecal samples. PSV amplicon (212 bp) was not detected in this age group. For nursery pigs, Porcine enteric picornaviruses amplicons were present in 77.8 % (14/18) of the faecal samples. PTV and EV-G were detected in single and mixed infections, while PSV was detected only in two samples in co-infection with PTV and EV-G in this age group. The Brazilian regions evaluated presented at least two of the tested viruses. Sequencing analysis revealed high similarities to the related viruses (95.3 to 99.2 % for PTV, 94.2 to 98.5 % for PSV and 86 to 100 % for EV-G). For the first time PTV, PSV and EV-G have been molecularly detected and characterised in pig faecal samples in Brazil.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig 1

Similar content being viewed by others

References

  • ABIPECS Brazilian Pork Industry and Exporter Association <http://www.abipecs.org.br/pt/estatisticas/mundial/producao-2.html> Accessed in: 28 July 2013

  • Alfieri, A.A., Parazzi, M.E., Takiuchi, E., Médici, K.C. and Alfieri, A.F., 2006. Frequency of group A rotavirus in diarrhoeic calves in Brazilian cattle herds, 1998–2002, Tropical Animal Health and Production, 38, 521--526

    Article  CAS  PubMed  Google Scholar 

  • Bangari, D.S., Pogranichniy, R.M., Gillespie, T. and Stevenson, G.W., 2010. Genotyping of porcine teschovirus from nervous tissue of pigs with and without polioencephalomyelitis in Indiana, Journal of Veterinary Diagnostic Investigation, 22, 594--597

    Article  PubMed  Google Scholar 

  • Boom, R., Sol, C.J., Salimans, M.M., Jansen, C.L., Wertheim-van Dillen, P.M. and van der Noordaa, J., 1990. Rapid and simple method for purification of nucleic acids, Journal of Clinical Microbiology, 28, 495--503

    CAS  PubMed Central  PubMed  Google Scholar 

  • Boros, Á., Nemes, C., Pankovics, P., Kapusinszky, B., Delwart, E. and Reuter, G., 2012. Porcine teschovirus in wild boars in Hungary, Archives of Virology, 157, 1573--1578

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Boros, Á., Pankovics, P. and Reuter, G., 2011. Characterization of a novel porcine enterovirus in domestic pig in Hungary, Infection, Genetics and Evolution, 11, 1096--1102

    Article  CAS  PubMed  Google Scholar 

  • Buitrago, D., Cano-Gómez, C., Agüero, M., Fernandez-Pacheco, P., Gómez-Tejedor, C. and Jiménez-Clavero, M.Á., 2010. A survey of porcine picornaviruses and adenoviruses in fecal samples in Spain, Journal of Veterinary Diagnostic Investigation, 22, 763--766

    Article  PubMed  Google Scholar 

  • Cano-Gómez, C., García-Casado, M.A., Soriguer, R., Palero, F. and Jiménez-Clavero, M.A., 2013. Teschoviruses and sapeloviruses in faecal samples from wild boar in Spain, Veterinary Microbiology, 165, 115--122

    Article  PubMed  Google Scholar 

  • Cano-Gómez, C., Palero, F., Buitrago, M.D., García-Casado, M.A., Fernández-Pinero, J., Fernández-Pacheco, P., Agüero, M., Gómez-Tejedor, C. and Jiménez-Clavero, M.Á., 2011. Analyzing the genetic diversity of teschoviruses in Spanish pig populations using complete VP1 sequences, Infection, Genetics and Evolution, 11, 2144--2150

    Article  PubMed  Google Scholar 

  • Deng, M.Y., Millien, M., Jacques-Simon, R., Flanagan, J.K., Bracht, A.J., Carrillo, C., Barrette, R.W., Fabian, A., Mohamed, F., Moran, K., Rowland, J., Swenson, S.L., Jenkins-Moore, M., Koster, L., Thomsen, B.V., Mayr, G., Pyburn, D., Morales, P., Shaw, J., Burrage, T., White, W., McIntosh, M.T. and Metwally, S., 2012. Diagnosis of Porcine teschovirus encephalomyelitis in the Republic of Haiti, Journal of Veterinary Diagnostic Investigation, 24, 671--678

    Article  PubMed  Google Scholar 

  • ICTV International Committee on Taxonomy of Viruses <http://ictvonline.org/virusTaxonomy.asp?version=2012> Accessed in: 03 May 2013

  • Kaku, Y., Murakami, Y., Sarai, A., Wang, Y., Ohashi, S. and Sakamoto, K., 2007. Antigenic properties of porcine teschovirus 1 (PTV-1) Talfan strain and molecular strategy for serotyping of PTVs, Archives of Virology, 152, 929--940

    Article  CAS  PubMed  Google Scholar 

  • Kaku, Y., Sarai, A. and Murakami, Y., 2001. Genetic reclassification of porcine enteroviruses, Journal of General Virology, 82, 417--424

    CAS  PubMed  Google Scholar 

  • Knowles, N.J., 1988. The association of group III porcine enteroviruses with epithelial tissue, Veterinary Record, 122, 441--442

    Article  CAS  PubMed  Google Scholar 

  • Knowles, N.J., 2006. Porcine enteric picornaviruses, In: Straw, B.E., Zimmerman, J.J., D’Allaire, S. and Taylor, D.J. (Eds.), Diseases of swine, 9th ed. (Blackwell Publishing, Ames, Iowa), p. 337--345

    Google Scholar 

  • Krumbholz, A., Dauber, M., Henke, A., Birch-Hirschfeld, E., Knowles, N.J., Stelzner, A. and Zell, R., 2002. Sequencing of porcine enterovirus groups II and III reveals unique features of both virus groups, Journal of Virology, 76, 5813--5821

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Krumbholz, A., Wurm, R., Scheck, O., Birch-Hirschfeld, E., Egerer, R., Henke, A., Wutzler, P. and Zell, R., 2003. Detection of porcine teschoviruses and enteroviruses by LightCycler real-time PCR, Journal of Virological Methods, 113, 51--63

    Article  CAS  PubMed  Google Scholar 

  • La Rosa, G., Muscillo, M., Di Grazia, A., Fontana, S., Iaconelli, M. and Tollis, M., 2006. Validation of RT-PCR assays for molecular characterization of porcine teschoviruses and enteroviruses, Journal of Veterinary Medicine, Series B, 53, 257--265

    Article  Google Scholar 

  • Lan, D., Ji, W., Yang, S., Cui, L., Yang, Z., Yuan, C. and Hua, X., 2011. Isolation and characterization of the first Chinese porcine sapelovirus strain, Archives of Virology, 156, 1567--1574

    Article  CAS  PubMed  Google Scholar 

  • Lin, W., Cui, S. and Zell, R., 2012. Phylogeny and evolution of porcine teschovirus 8 isolated from pigs in China with reproductive failure, Archives of Virology, 157, 1387--1391

    Article  CAS  PubMed  Google Scholar 

  • Moon, H.-J., Song, D., Seon, B.H., Kim, H.-K., Park, S.-J., An, D.-J., Kim, J.-M., Kang, B.-K. and Park, B.-K., 2012. Complete genome analysis of porcine enterovirus B isolated in Korea, Journal of Virology, 86, 10250

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Prodělalová, J., 2012. The survey of porcine teschoviruses, sapeloviruses and enteroviruses B infecting domestic pigs and wild boars in the Czech Republic between 2005 and 2011, Infection, Genetics and Evolution, 12, 1447--1451

    Article  PubMed  Google Scholar 

  • Salles, M.W.S., Scholes, S.F.E., Dauber, M., Strebelow, G., Wojnarowicz, C., Hassard, L., Acton, A.C. and Bollinger, T.K., 2011. Porcine teschovirus polioencephalomyelitis in western Canada, Journal of Veterinary Diagnostic Investigation, 23, 367--373

    Article  PubMed  Google Scholar 

  • Shan, T., Li, L., Simmonds, P., Wang, C., Moeser, A. and Delwart, E., 2011. The fecal virome of pigs on a high-density farm, Journal of Virology, 85, 11697--11708

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Sozzi, E., Barbieri, I., Lavazza, A., Lelli, D., Moreno, A., Canelli, E., Bugnetti, M. and Cordioli, P., 2010. Molecular characterization and phylogenetic analysis of VP1 of porcine enteric picornaviruses isolates in Italy, Transboundary and Emerging Diseases, 57, 434--442

    Article  CAS  PubMed  Google Scholar 

  • Ventura, A., Gonzalez, W., Barrette, R., Swenson, S., Bracht, A., Rowland, J., Fabian, A., Moran, K., Mohamed, F., O’Hearn, E., Jenkins-Moore, M., Toms, D., Shaw, J., Morales, P., Pyburn, D., Carrillo, C., Mayr, G., McIntosh, M. and Deng, M., 2013. Virus and antibody diagnostics for swine samples of the Dominican Republic collected in regions near the border to Haiti, ISRN Virology, 2013, 1--7

    Article  Google Scholar 

  • Zell, R., Dauber, M., Krumbholz, A., Henke, A., Birch-Hirschfeld, E., Stelzner, A., Prager, D. and Wurm, R., 2001. Porcine teschoviruses comprise at least eleven distinct serotypes: molecular and evolutionary aspects, Journal of Virology, 75, 1620--1631

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Zell, R., Krumbholz, A., Henke, A., Birch-Hirschfeld, E., Stelzner, A., Doherty, M., Hoey, E., Dauber, M., Prager, D. and Wurm, R., 2000. Detection of porcine enteroviruses by nRT–PCR: differentiation of CPE groups I–III with specific primer sets, Journal of Virological Methods, 88, 205--218

    Article  CAS  PubMed  Google Scholar 

  • Zhang, W., Yang, S., Shen, Q., Ren, L., Shan, T., Wei, J., Cui, L. and Hua, X., 2012. Complete genome sequence of a novel porcine enterovirus strain in China, Journal of Virology, 86, 7008–7009

    Article  CAS  PubMed Central  PubMed  Google Scholar 

Download references

Acknowledgments

The authors are extremely grateful to Gefferson Almeida da Silva and Angelica Schmeing for their technical collaboration. We would like to thank the following Brazilian Institutes for financial support: the National Counsel of Scientific and Technological Development (CNPq), the Brazilian Federal Agency for Support and Evaluation of Graduate Education (CAPES), Financing of Studies and Projects (FINEP), and the Araucária Foundation (FAP/PR). Alfieri A.A. and Alfieri, A.F. are recipients of CNPq fellowships.

Author information

Authors and Affiliations

  1. Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid—Campus Universitário, 86057-970, Londrina, PR, Brazil, PO Box 10011

    Daiane Güllich Donin, Raquel de Arruda Leme, Alice Fernandes Alfieri & Amauri Alcindo Alfieri

  2. Department of Veterinary Medicine, Universidade Federal do Paraná, Palotina, Paraná, Brazil

    Geraldo Camilo Alberton

Authors
  1. Daiane Güllich Donin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Raquel de Arruda Leme
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Alice Fernandes Alfieri
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Geraldo Camilo Alberton
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Amauri Alcindo Alfieri
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Amauri Alcindo Alfieri.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Donin, D.G., de Arruda Leme, R., Alfieri, A.F. et al. First report of Porcine teschovirus (PTV), Porcine sapelovirus (PSV) and Enterovirus G (EV-G) in pig herds of Brazil. Trop Anim Health Prod 46, 523–528 (2014). https://doi.org/10.1007/s11250-013-0523-z

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11250-013-0523-z

Keywords

Search

Navigation