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Effects of the Administration of Lactobacilli, Maltodextrins and Fructooligosaccharides upon the Adhesion of E. coli O8:K88 to the Intestinal Mucosa and Organic Acid Levels in the Gut Contents of Piglets

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Abstract

The influence of the administration of Lactobacillus plantarum, maltodextrin Maldex 150 and Raftifeed IPX fructooligosaccharides on the inhibition of adhesion of E. coli O8:K88 to the mucosa of the jejunum, ileum and colon as well as on the organic acid levels was investigated in 33 conventional piglets. The counts of E. coli K88 adhering to the jejunal mucosa were significantly decreased (p < 0.05) in Lact. plantarum + Maldex 150 and Lact. plantarum + Maldex 150 + Raftifeed IPX groups. The counts of E. coli K88 adhering to the colonic mucosa of Lact. plantarum + Maldex 150 + Raftifeed IPX and Lact. plantarum + Raftifeed IPX groups were significantly lower (p < 0.05) than in Lact. plantarum and Lact. plantarum + Maldex 150 animals. The acetic acid levels in the ileum and colon of the Lact. plantarum + Maldex 150 + Raftifeed IPX group and Lact. plantarum + Raftifeed IPX group were significantly higher (p < 0.05) than in the Lact. plantarum and Lact. plantarum + Maldex 150 group. The combination of Lact. plantarum, maltodextrin Maldex 150 and Raftifeed IPX proved to be the most effective one to inhibit the counts of E. coli O8:K88 adhering to the intestinal mucosa of the jejunum and colon of conventional piglets.

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Abbreviations

cfu:

colony-forming units

Lact. plantarum :

Lactobacillus plantarum

MRS:

de Man – Rogosa – Sharpe broth

PBS:

phosphate-buffered saline

TLC:

thin-layer chromatography

References

  • Bailey, J.S., Blankenship, L.C. and Cox, N.A., 1991. Effect of fructooligosaccharide on Salmonella colonization of the chicken intestine. Poultry Science, 70, 2433–2438

    PubMed  CAS  Google Scholar 

  • Bekaert, H., Moermans, R. and Eeckhout, W., 1996. Influence d'une culture de levure vivante (Levucell SB2) dans un aliment pour porcelets sevrés sur les performances zootechniques et sur la fréquence des diarrhées. Annales de Zootechnie, 45, 369–376

    Google Scholar 

  • Bomba, A., Gancarčíková, S., Nemcová, R., Herich, R., Kaštel', R., Depta, A., Demeterová, M., Ledecký, V. and Žitňan, R., 1998. The effect of lactic acid bacteria on intestinal metabolism and metabolic profile of gnotobiotic pigs. Deutsche Tierarztliche Wochenschrift, 105, 384–389

    PubMed  CAS  Google Scholar 

  • Bomba, A., Nemcová, R., Gancarčíková, S., Herich, R. and Kaštel', R., 1999. Potentiation of the effectiveness of Lactobacillus casei in prevention of E. coli induced diarrhea in conventional and gnotobiotic pigs. In: P.S. Paul, D.H. Francis (eds), Mechanisms in the Pathogenesis of Enteric Diseases 2, (Kluwer Academic/Plenum Publishers, New York), 185–190

    Google Scholar 

  • Bomba, A., Nemcová, R., Mudroňová, D. and Guba, P., 2002. The possibilities of potentiating the efficacy of probiotics. Trends in Food Science and Technology, 13, 121–126

    Article  CAS  Google Scholar 

  • Bomba, A., Nemcová, R., Gancarčíková, S., Herich, R., Pistl, J., Revajová, V., Jonecová, Z., Bugarský, A., Levkut, M., Kaštel', R., Baran, M., Lazar, G., Hluchý, M., Maršálková, S. and Pošivák, J., 2003. The influence of ω-3 polyunsaturated fatty acids on lactobacilli adhesion to the intestinal mucosa and on immunity in gnotobiotic piglets. Berliner und Munchener Tierarztliche Wochenschrift, 116, 312–316

    PubMed  CAS  Google Scholar 

  • Bury, D., Jelen, P. and Kimura, K., 1998. Whey protein concentrate as a nutrient supplement for lactic acid bacteria. International Dairy Journal, 8, 149–151

    Article  CAS  Google Scholar 

  • Crittenden, R.G., 1999. Prebiotics. In: G.W. Tannock (ed.), Probiotics: A Critical Review (Horizon Scientific Press, Wymondham), 141–156

    Google Scholar 

  • DeCupere, F., Deprez, P., Demeulenaere, D. and Muylle, E., 1992. Evaluation of the effect of 3 probiotics on experimental Escherichia coli enterotoxaemia in weaned piglets. Journal of Veterinary Medicine B, 39, 277–284

    Article  CAS  Google Scholar 

  • Depta, A., Rychlik, R., Nieradka, R., Rotkiewicz, T., Kujawa, K., Bomba, A. and Grabowska-Świecicka, G., 1998. The influence of alimentary tract colonization with Lactobacillus sp. strains on chosen metabolic profile indices in piglets. Polish Journal of Veterinary Science, 1–2, 3–7

    Google Scholar 

  • Gibson, G.R. and Fuller, R., 2002. Symposium: probiotic bacteria: Implications for human health. Aspects of in vitro and in vivo research approaches directed toward identifying probiotics and prebiotics for human use. Journal of Nutrition, 130, 391–395

    Google Scholar 

  • Gibson, G.R. and Roberfroid, M.B., 1995. Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. Journal of Nutrition, 125, 1401–1412

    PubMed  CAS  Google Scholar 

  • Kimura, N., Yoshikane, M., Kobayashi, A. and Mitsuoka, T., 1983. An application of dried bifidobacteria preparation to scouring animals. Bifido Microflora, 2, 41–55

    Google Scholar 

  • Maeng, W.J., Kim, C.W. and Shin, H.T., 1989. Effect of feeding lactic acid bacteria concentrate (LBC, Streptococcus faecium Cernelle 68) on the growth rate and prevention of scouring in piglets. Korean Journal of Animal Science, 31, 318

    Google Scholar 

  • Marchal, L.M., Beeftink, H.H. and Tramper, J., 1999. Towards a rational design of commercial maltodextrins. Trends in Food Science and Technology, 10, 345–355

    Article  CAS  Google Scholar 

  • Nakakuki, T., 1993. Oligosaccharides. Production, properties and applications. Japanese Technological Review, vol. 3, no. 2, (Gordon and Breach Science Publishers, London)

    Google Scholar 

  • Nemcová, R., 1998. In vitro utilizácia fruktooligosacharidov a inhibícia E. coli 08:K88ab:H9 laktobacilmi. Veterinárske správy, 12, 18–23

    Google Scholar 

  • Nemcová, R., Lauková, A., Gancarčíková, S. and Kaštel', R., 1997. In vitro studies of porcine lactobacilli for possible probiotic use. Berliner und Munchener Tierarztliche Wochenschrift, 110, 413–417

    PubMed  Google Scholar 

  • Nemcová, R., Bomba, A., Gancarčíková, S., Herich, R. and Guba, P., 1999. Study of the effect of Lactobacillus paracasei and fructooligosaccharides on the faecal microflora in weanling piglets. Berliner und Munchener Tierarztliche Wochenschrift, 112, 225–228

    PubMed  Google Scholar 

  • Nousiainen, J. and Setälä, J., 1993. Lactic acid bacteria as animal probiotics. In: S. Salminen and A. Wright (eds), Lactic Acid Bacteria, (Marcel Dekker, New York), 315–356

    Google Scholar 

  • Simon, O. and Jadamus, A., 2002. Probiotics and prebiotics. Proceedings of the 11th European Poultry Conference, 6–10 September, Bremen, Germany, 45–46

  • Yadava, J.N.S., Gupta, S., Ahmad, I., Varma, N. and Tandon, J.S., 1995. Neutralization of enterotoxins of Eschericha coli by coleonol (forskolin) in rabbit and guinea pig ileal loop models. Indian Journal of Animal Science, 65, 1177–1181

    CAS  Google Scholar 

  • Zimmerman, B., Bauer, E. and Mosenthin, R., 2001. Pro- and prebiotics in pig nutrition—potential modulators of gut health? Journal of Animal Feed Science, 10, 47–56

    Google Scholar 

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Authors and Affiliations

  1. University of Veterinary Medicine, Košice, Slovak Republic

    R. Nemcová, A. Bomba, S. Gancarčíková, P. Guba, J. Koščová, Z. Jonecová, L. Sciranková & A. Bugarský

  2. Institute of Chemical Sciences, P.J. Šafárik University, Košice, Slovak Republic

    K. Reiffová

Authors
  1. R. Nemcová
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  2. A. Bomba
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  3. S. Gancarčíková
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  4. K. Reiffová
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  5. P. Guba
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  6. J. Koščová
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  7. Z. Jonecová
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  8. L. Sciranková
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  9. A. Bugarský
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Correspondence to R. Nemcová.

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Nemcová, R., Bomba, A., Gancarčíková, S. et al. Effects of the Administration of Lactobacilli, Maltodextrins and Fructooligosaccharides upon the Adhesion of E. coli O8:K88 to the Intestinal Mucosa and Organic Acid Levels in the Gut Contents of Piglets. Vet Res Commun 31, 791–800 (2007). https://doi.org/10.1007/s11259-007-0048-x

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  • DOI: https://doi.org/10.1007/s11259-007-0048-x

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