Abstract
The hemagglutinin (HA) genes of 12 H9N2 influenza virus strains isolated from chickens in Mainland China during the period 1995–2002 were genetically analyzed. All the isolates possessed the same amino acid motif -R-S-S-R/G-L- at the cleavage site of HA. Except for the conserved amino acids, as is the case in the other avian influenza viruses, located in the receptor binding site, all of the 12 isolates possessed N at amino acid position 183; A, T, or V at position 190; K at position 137, whereas the representative strains of the other lineage (except Dk/HK/Y280/97-like lineage) virus of H9N2 viruses had H, E, and R at these positions respectively. These could be considered as the partial molecular markers of the H9 viruses isolated from chickens in Mainland China. Phylogenetic analyses showed HA genes of these isolates belonged to that of A/duck/Hong Kong/Y280/97-like virus lineage. No A/quail/Hong Kong/Gl/97-like virus was found in chicken, population since the outbreak of H9N2 influenza in Mainland China in 1992. The available evidence indicates that HA genes of H9 influenza virus circulating in Mainland China during the past years were well conserved.
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Lee C.W., Song C.S., Lee Y.J., Mo I.P., Garcia M., Suarez D.L., and Kirn S.J. Avian Dis 44, 527–535, 2000.
Naeem K., Ullah A, Manvell R.J., and Alexander D.J., Vet Rec 145, 560, 1999.
Tang X.Y., Tian G.B., Zhao C.S., Zhou J.F., and Yu K.Z., Chin J Pre Vet Med 1, 1–5, 1998.
Guan Y., Shortridge K.F., Krauss S., and Webster R.G., Proc Natl Acad Sci USA 96, 9363–9367, 1999.
Lin Y.P., Shaw M., Gregory V., Cameron K., Lim W., Klimov A., Subbarao K., Guan Y., Krauss S., Shortridge K., Webster R., Cox N., and Hay A., Proc Natl Acad Sci USA 97, 9654–9358, 2000.
Cameron K.R., Gregory V., Banks J., Brown L.H., Alexander D.J., Hay A.J., and Lin Y.P., Virology 278, 36–41, 2000.
Lee C.W., Song C.S., Lee Y.J., Mo I.P., Garcia M., Suarez D.L., and Kim S.J., Avian Dis 44, 527–535, 2000.
Guan Y., Shortridge K.F., Krauss S., Chin P.S., Dyrting K.C., Ellis T.M., Webster R.G., and Peiris M., J Virol 74, 9372–9380, 2000.
Liu J.H., Okazaki K., Ozaki H., Sakoda Y., Wu Q.M., Chen F.Y., and Kida H., Avian Pathol 32, 551–560, 2003.
Kendal A.P., Pereira M.S., and Skehel J.J., Concepts and procedures for laboratory-based influenza surveillance, U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, Atlanta, GA, 1982.
Shu L.L., Lin Y.P., Wright S.M., Shortridge K.F., and Webster R.G., Virology 202, 825–833, 1994.
Kawaoka Y., and Webster R.G., Proc Natl Acad Sci USA 85, 324–328, 1988.
Matrosovich M., Zhou N., Kawaoka Y., and Webster R.G., J Virol 73, 1146–1155, 1999.
Guo Y.J., Krauss S., Senne D.A., Mo I.P., Lo K.S., Xiong X.P., Norwood M., Shortridge K.F., Webster R.G., and Guan Y., Virology 267, 279–288, 2000.
Weis W., Brown J.H., Cusack S., Paulson J.C., Skehel J.J., and Wiley D.C., Nature 333, 426–431, 1988.
Nobusawa E., Aoyama T., Kato H., Suzuki Y., Tateno Y., and Nakajima K., Virology 182, 475–485, 1991.
Liu H.Q., Liu X.F., Cheng J.A., Peng D.X., Jia L.J., and Huang Y., Avian Dis 47, 116–127, 2003.
Matrosovich M., Tuzikov A., Bovin N., Gambaryan A., Klimov A., Castrucci M.R., Donatelli I., and Kawaoka Y., J Virol 74, 8502–8512, 2000.
Matrosovich M.N., Krauss S., and Webster R.G., Virology 281, 156–162, 2001.
Gambaryan A., Webster R., and Matrosovich M., Arch Virol 147, 1197–1208, 2002.
Liu M., He S., Walker D., Zhou N., Perez D.R., Mo B., Li F., Huang X., Webster R.G., and Webby R.J., Virology 20, 267–275, 2003.
Li K.S., Xu K.M., Peiris J.S.M., Poon L.L.M., Yu K.Z., Yuen K.Y., Shortridge K.F., Webster R.G., and Guan Y.J., Virol 77, 6988–6994, 2003.
Liu J.H., Okazaki K., Shi W.M., Wu Q.M., Mweene A.S., and Kida H., Virus Genes 27, 197–202, 2003.
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liu, JH., Okazaki, K., Mweene, A. et al. Genetic Conservation of Hemagglutinin Gene of H9 Influenza Virus in Chicken Population in Mainland China. Virus Genes 29, 329–334 (2004). https://doi.org/10.1007/s11262-004-7436-x
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DOI: https://doi.org/10.1007/s11262-004-7436-x