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Genomic Analysis of Recombinant Sabin Clinical Isolates

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Abstract

Recombination in Poliovirus vaccine strains is a very frequent phenomenon. In this report 23 polio/Sabin strains isolated from healthy vaccinees or from VAPP patients after OPV administration, were investigated in order to identify recombination sites from 2C to 3D regions of the poliovirus genome. RT-PCR, followed by Restriction Fragment Length Polymorphism (RFLP) screening analysis were applied in four distant genomic regions (5′ UTR, VP1, 2C and 3C–3D) in order to detect any putative recombinant. The detected recombinants were sequenced from 2C to the end of the genome (3′ UTR) and the exact recombination sites were determined with computational analysis. Five of the 23 isolated strains were recombinant in one genomic region, two of them in 2C, isolates EP16:S3/S2, EP23:S3/S1, two in 3D isolates EP6:S2/S1, EP12:S2/S1 and one in 3A isolate EP9:S2/Sl. Point mutations were found in strains EP3, EP6, EP9 and EP12. Recombination specific types and sites re-occurrence along with point mutations are discussed concerning the polioviruses evolution.

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Acknowledgements

The present work was co funded by the European Union (75%) and the Greek Ministry of Education (25%) under the framework of the Education and Initial Vocational Training Program “Archimedes”. Research project, “potency testing of trivalent oral poliovirus (OPV) vaccine”.

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Authors and Affiliations

  1. Department of Biochemistry and Biotechnology, School of Health Sciences, University of Thessaly, Ploutonos 26 and Aiolou, 41221, Larissa, Greece

    Eleni Paximadi, Zissis Mamuris, Constantinos Stathopoulos & Panayotis Markoulatos

  2. Virology Laboratory, Hellenic Pasteur Institute, Athens, Greece

    Eleni Paximadi

  3. School of Animal and Microbial Sciences, University of Reading, Reading, UK

    Ioannis Karakasiliotis

  4. Technological Educational Institute of Larissa, Larissa, Greece

    Vassilis Krikelis

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  1. Eleni Paximadi
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  2. Ioannis Karakasiliotis
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  3. Zissis Mamuris
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  4. Constantinos Stathopoulos
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  5. Vassilis Krikelis
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  6. Panayotis Markoulatos
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Correspondence to Panayotis Markoulatos.

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Paximadi, E., Karakasiliotis, I., Mamuris, Z. et al. Genomic Analysis of Recombinant Sabin Clinical Isolates. Virus Genes 32, 203–210 (2006). https://doi.org/10.1007/s11262-005-6877-1

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  • DOI: https://doi.org/10.1007/s11262-005-6877-1

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