Abstract
A part of the nucleotide sequence of the 5′ untranslated region (5′UTR) and Erns region, and the genomic regions encoding for Npro, Core, and E2 of So-like isolates and IS25CP/01 strain which belong to bovine viral diarrhea virus genotype 1 (BVDV-1) were determined and genetic comparisons were made with sequences of other BVDV subgenotypes. Phylogenetic analysis using the 5′UTR and Npro revealed that So-like isolates and IS25CP/01 branched into independent phylogenetic branch. So-like isolates were clustered with Korean BVDV strains taken from DDBL/EMBL/GenBank in the 5′UTR. An additional two amino acid residues were found at the C terminal of the Core region of IS25CP/01. The similarity of amino acid sequence of E2 of So-like isolates and IS25CP/01 to the BVDV-1 reference strain NADL were 78.0–78.5 and 79.0, respectively. Cross-neutralization tests showed significant antigenic differences between So-like isolates and the others (Antigenic similarity (R) value: 2.2–8.8), and IS25CP/01 and the others (R value: 1.6–8.8). So-like viruses and IS25CP/01 differed from the thirteenth subgenotypes (1a-1m) reported by Jackova et al. (2007) and were classified as new genetic subtypes, BVDV-1n (So-like) and 1o (IS25CP/01).
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Acknowledgments
We are thankful to the Hokkaido and Aichi prefectural livestock hygiene service center for providing BVDV isolates. We also thank Ms. H. Shibuya and Dr. M. Tajima for technical help with the genetic analysis and critical review of the manuscript.
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The nucleotide sequence data reported in this article have been submitted to the DDBJ/EMBL/GenBank nucleotide sequence database and assigned the accession numbers AB359923–AB359944.
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Nagai, M., Hayashi, M., Itou, M. et al. Identification of new genetic subtypes of bovine viral diarrhea virus genotype 1 isolated in Japan. Virus Genes 36, 135–139 (2008). https://doi.org/10.1007/s11262-007-0190-0
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DOI: https://doi.org/10.1007/s11262-007-0190-0