Abstract
The nucleotide sequence of Pineapple mealybug wilt associated virus-3 (PMWaV-3) (Closteroviridae: Ampelovirus), spanning seven open reading frames (ORFs) and the untranslatable region of the 3′ end was determined. Based on the amino acid identities with orthologous ORFs of PMWaV-1 (54%–73%) and PMWaV-2 (13%–35%), we propose PMWaV-3 is a new species in the PMWaV complex. PMWaV-3 lacks an intergenic region between ORF1b and ORF2, encodes a relatively small, 28.8 kDa, coat protein, and lacks a coat protein duplicate. Phylogenetic analyses were used to analyze seven different domains and ORFs from members of the family Closteroviridae. Two distinct clades within the recognized genus Ampelovirus were observed; one that includes PMWaV-3 and PMWaV-1 and several GLRaVs and another that includes PMWaV-2 and GLRaV-3, the type member of the genus Ampelovirus.
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Acknowledgments
This research funded, in part, by a Grant from the Hawaii Department of Agriculture (Contract No. 49048), a Grant through the Tropical/Subtropical Agricultural Research Program (Project No. HAW00985-10145), a grant from the United States Department of Agriculture-Cooperative State Research, Education, and Extension Service (USDA-CSREES) Grants Program-Pest Management Alternatives Program (Project No. HAW00937-G under agreement 2003-34381-13659), a Grant from the USDA-CSREES Regional Integrated Pest Management Competitive Grants Program-Western Region (Project No. Haw01951-G under agreement 2006-34103-17176).
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Sether, D.M., Melzer, M.J., Borth, W.B. et al. Genome organization and phylogenetic relationship of Pineapple mealybug wilt associated virus-3 with family Closteroviridae members. Virus Genes 38, 414–420 (2009). https://doi.org/10.1007/s11262-009-0334-5
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DOI: https://doi.org/10.1007/s11262-009-0334-5