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Genomic characterization of the first class I Newcastle disease virus isolated from the mainland of China

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An Erratum to this article was published on 13 March 2010

Abstract

The complete genomic sequence of Newcastle disease virus (NDV) strain NDV08-004, isolated from domestic ducks in China, was determined in this study. The genome is 15198 nucleotides (nt) in length, follows the “rule of six” and contains a 55-nt leader sequence at the 3′ end and a 114-nt trailer sequence at the 5′ end. Compared with the full genome sequences of Class II NDV strains, the NDV08-004 isolate has a 12-nt insertion (TGGGAGACGGGG) in the phosphoprotein gene between nucleotides 2381 and 2382 of the genome (numbered according to the genomic sequence of the La Sota strain, which consists of 15186 nt). Strain NDV08-004 has the motif 112E-Q-Q-E-R-L117 at the cleavage site of the fusion protein, which is typical of lentogenic NDV strains, and this is in agreement with the results of pathogenic tests based on the mean death time (MDT) and the intracerebral pathogenicity index (ICPI). Phylogenetic analysis based on the full genome revealed that all the NDV strains studied could be divided into two distinct clades, namely class I and class II, and the NDV08-004 isolate characterized in this study was grouped in class I. Further phylogenetic analysis based on a 374-bp fragment of the F gene in class I strains of NDV demonstrated that NDV08-004 belongs to genotype 3, and should be therefore similar to strains obtained from live bird markets in Hong Kong in recent years.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant number 30901079).

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Authors and Affiliations

  1. National Reference Laboratory for Newcastle Disease, China Animal Health and Epidemiology Center, Qingdao, 266032, China

    Hualei Liu, Fei Chen, Yunling Zhao, Dongxia Zheng, Jinming Li, Tiangang Xu & Zhiliang Wang

  2. School of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, China

    Lin Qi

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  1. Hualei Liu
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  2. Fei Chen
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  3. Yunling Zhao
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  4. Dongxia Zheng
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  5. Jinming Li
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  8. Zhiliang Wang
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Correspondence to Zhiliang Wang.

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Hualei Liu and Fei Chen contributed equally to this paper.

An erratum to this article can be found at http://dx.doi.org/10.1007/s11262-010-0471-x

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Liu, H., Chen, F., Zhao, Y. et al. Genomic characterization of the first class I Newcastle disease virus isolated from the mainland of China. Virus Genes 40, 365–371 (2010). https://doi.org/10.1007/s11262-010-0452-0

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