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An RNA-dependent RNA polymerase gene of a distinct Brazilian tospovirus

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Abstract

The tospoviral RNA-dependent RNA polymerases (RdRp), or L proteins, perform several conserved functions during virus replication in host cells. In this study, an L segment sequence of 9,040 bp from a new tospovirus (family Bunyaviridae) naturally infecting bean (Phaseolus vulgaris L.) plants was characterized. It encodes the largest RdRp gene known yet for this genus, with deduced 2932aa and a molecular mass of approximately 336 kDa. A Lysine-rich C-terminal extension was found, which apart from our isolate, was only recognized in another recently discovered tospovirus infecting Fabaceae, Soybean vein necrosis associated virus (SVNaV). Due to its distinct biological features and L protein-based phylogenetic analysis showing an almost equidistant position in comparison to Eurasian and American Tospovirus groups, as well as the clustering with SVNaV, we suggest the tentative name Bean necrotic mosaic virus for this unique isolate.

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Acknowledgment

Authors would like to thank CNPq (Conselho Nacional de Desenvolvimento Tecnológico) and Capes (Conselho de Aperfeiçoamento de Pessoal de Nível Superior) for financial support and Embrapa Vegetables technicians Lúcio Flávio and Hamilton for greenhouse assistance.

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Authors and Affiliations

  1. Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, 70910-900, Brazil

    Athos Silva de Oliveira, André Gustavo Machado Bertran, Tatsuya Nagata & Renato Oliveira Resende

  2. Embrapa Hortaliças, Caixa Postal 218, Brasília, DF, 70359-970, Brazil

    Alice Kazuko Inoue-Nagata

  3. Departamento de Entomologia, Fitopatologia e Zoologia Agrícola, Escola Superior de Agricultura Luiz de Queiroz, Caixa Postal 9, Piracicaba, SP, 13418-900, Brazil

    Elliot Watanabe Kitajima

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  1. Athos Silva de Oliveira
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  2. André Gustavo Machado Bertran
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  3. Alice Kazuko Inoue-Nagata
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  4. Tatsuya Nagata
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  5. Elliot Watanabe Kitajima
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  6. Renato Oliveira Resende
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Correspondence to Renato Oliveira Resende.

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de Oliveira, A.S., Bertran, A.G.M., Inoue-Nagata, A.K. et al. An RNA-dependent RNA polymerase gene of a distinct Brazilian tospovirus. Virus Genes 43, 385–389 (2011). https://doi.org/10.1007/s11262-011-0639-z

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  • DOI: https://doi.org/10.1007/s11262-011-0639-z

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